Document Detail


Rat abdominal aorta stenting: a new and reliable small animal model for in-stent restenosis.
MedLine Citation:
PMID:  15375325     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A high throughput animal model may enhance pathophysiological studies to mechanisms of in-stent restenosis (ISR). More and appropriate antibodies and transgenic and knockout strains are available in rats. Consequently, a model for ISR in the rat would be convenient for pathobiological studies. Here we present the full characteristics of a rat ISR model suitable for high throughput stent research. METHODS: The abdominal aorta of rats was separated from surrounding tissue and a BeStenttrade mark 2 or a Cyphertrade mark sirolimus-eluting stent was locally inserted. After 1, 3, 7, 28 and 56 days, the aortas were harvested, fixed, embedded and cut. Morphometric analysis was performed and inflammation scored. RESULTS: The neointimal area increased to a maximum after 28 days (0.55 +/- 0.08 mm(2)). Subsequently, the neointimal area slightly decreased. The injury score and the neointimal area were linearly correlated (r = 0.85, p < 0.01). Thrombus formation was present after 1 day. Leukocyte adherence was evident after 1 day, maximal after 3 days (93 +/- 21 cells/section) and decreased thereafter. The inflammation score increased after 3 days to a maximum after 7 days (1.37 +/- 0.06) and declined thereafter. After 28 days the Cypher sirolimus-eluting stent decreased the stenosis in comparison to the BeStent 2 (10.2 +/- 0.85 vs. 18.0 +/- 2.0%, respectively, p < 0.01). CONCLUSIONS: Stent deployment in the rat abdominal aorta results in thrombus formation, inflammation and neointimal formation. Moreover, there is a linear correlation between the injury score and the neointimal area. These responses resemble ISR events as seen in other animal models. Moreover, a known anti-restenotic stent also reduces neointimal formation in this model. Rat abdominal aorta stenting is a promising animal model for ISR, it is suitable for testing commercially manufactured stents and studying the pathophysiology of ISR.
Authors:
Bas Langeveld; Anton J M Roks; René A Tio; Ad J van Boven; Johannes J L van der Want; Rob H Henning; Heleen M M van Beusekom; Willem J van der Giessen; Felix Zijlstra; Wiek H van Gilst
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-09-15
Journal Detail:
Title:  Journal of vascular research     Volume:  41     ISSN:  1018-1172     ISO Abbreviation:  J. Vasc. Res.     Publication Date:    2004 Sep-Oct
Date Detail:
Created Date:  2004-11-23     Completed Date:  2004-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  377-86     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, University Hospital Groningen, Groningen, The Netherlands. be.langeveld@med.rug.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Abdominal / pathology,  physiopathology
Disease Models, Animal*
Graft Occlusion, Vascular*
Male
Rats
Rats, Wistar*
Reproducibility of Results
Specific Pathogen-Free Organisms
Stents*
Thrombosis / pathology,  physiopathology*
Tunica Intima / pathology,  physiopathology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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