Document Detail


Rat Sertoli cells express epithelial but also mesenchymal genes after immortalization with SV40.
MedLine Citation:
PMID:  15716129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new immortal Sertoli cell line from pubertal rat testis was established and characterized. We have generated the clonal line SCIT-C8 expressing established markers for Sertoli cells (SC) like transferrin, clusterin and steel factor/stem cell factor (SCF). Additionally, the immortalized cells express afadin, a protein which is a member of tight and adherens junctions, therefore the cells may be useful for studies of the blood-testis barrier (BTB) in vitro. In contrast to primary SC, the immortalized cells lost expression of androgen receptor and responsiveness to androgens and follicle-stimulating hormone. Surprisingly, we found mRNA expression and protein secretion of the mesenchymal markers, fibronectin and entactin-1, which we also observed for the immortalized SC lines, ASC-17D and 93RS2. In comparison to primary SC, the immortalized cells demonstrated enhanced adhesion in vitro. This correlated with the expression of entactin-1 because adhesion was strongly reduced by antibody perturbation experiments. Additionally, we found the alternatively spliced and primarily muscle cell-specific long variant of TGF-beta2 not only in peritubular cells (PC), but also in the primary and immortalized SC. Furthermore, all immortalized cell lines secreted higher amounts of TGF-beta2 than primary SC. In conclusion, the immortalized SC lines from different developmental stages showed a similar pattern of epithelial and mesenchymal markers.
Authors:
Lutz Konrad; Marcel Munir Keilani; Andrea Cordes; Elke V?lck-Badouin; Leslie Laible; Martin Albrecht; Heiner Renneberg; Gerhard Aum?ller
Related Documents :
11784049 - Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells hav...
15243649 - Flow cytometry based techniques to study testicular acidophilic granulocytes from the p...
2886439 - The rat testis produces large amounts of an interleukin-1-like factor.
20654509 - The co-culture of sertoli cells and germ cells: applications in toxicology.
6286509 - The deleterious effect of mechanical dissociation of rat testes on the functional activ...
2865929 - Ultrastructure of the interstitial cells of leydig, stimulated and unstimulated.
17030849 - The cr4 region of ebna2 confers viability of epstein-barr virus-transformed b cells by ...
20019239 - Glucose restriction can extend normal cell lifespan and impair precancerous cell growth...
1821619 - Morphological and immunological heterogeneity of human ovarian neoplasms.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-18
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1722     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-04-12     Completed Date:  2005-04-28     Revised Date:  2010-04-12    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  6-14     Citation Subset:  IM    
Affiliation:
Department of Urology, Uniklinikum Lahnberge, Baldingerstr, D-35033 Marburg, Germany. Konrad@post.med.uni-marburg.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Polyomavirus Transforming* / genetics,  metabolism
Cell Line, Transformed*
Epithelium / physiology*
Extracellular Matrix / chemistry
Fibronectins / genetics,  metabolism
Male
Membrane Glycoproteins / genetics,  metabolism
Mesoderm / physiology*
Rats
Sertoli Cells / cytology*,  physiology*
Testis / cytology
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Fibronectins; 0/Membrane Glycoproteins; 0/nidogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Formation of an oxo-radical of peroxovanadate during reduction of diperoxovanadate with vanadyl sulf...
Next Document:  Enhanced catalytic and conformational stability of Atlantic cod trypsin upon neoglycosylation.