| Rat Sertoli cells express epithelial but also mesenchymal genes after immortalization with SV40. | |
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MedLine Citation:
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PMID: 15716129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new immortal Sertoli cell line from pubertal rat testis was established and characterized. We have generated the clonal line SCIT-C8 expressing established markers for Sertoli cells (SC) like transferrin, clusterin and steel factor/stem cell factor (SCF). Additionally, the immortalized cells express afadin, a protein which is a member of tight and adherens junctions, therefore the cells may be useful for studies of the blood-testis barrier (BTB) in vitro. In contrast to primary SC, the immortalized cells lost expression of androgen receptor and responsiveness to androgens and follicle-stimulating hormone. Surprisingly, we found mRNA expression and protein secretion of the mesenchymal markers, fibronectin and entactin-1, which we also observed for the immortalized SC lines, ASC-17D and 93RS2. In comparison to primary SC, the immortalized cells demonstrated enhanced adhesion in vitro. This correlated with the expression of entactin-1 because adhesion was strongly reduced by antibody perturbation experiments. Additionally, we found the alternatively spliced and primarily muscle cell-specific long variant of TGF-beta2 not only in peritubular cells (PC), but also in the primary and immortalized SC. Furthermore, all immortalized cell lines secreted higher amounts of TGF-beta2 than primary SC. In conclusion, the immortalized SC lines from different developmental stages showed a similar pattern of epithelial and mesenchymal markers. |
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Authors:
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Lutz Konrad; Marcel Munir Keilani; Andrea Cordes; Elke V?lck-Badouin; Leslie Laible; Martin Albrecht; Heiner Renneberg; Gerhard Aum?ller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-11-18 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1722 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-04-12 Completed Date: 2005-04-28 Revised Date: 2010-04-12 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 6-14 Citation Subset: IM |
Affiliation:
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Department of Urology, Uniklinikum Lahnberge, Baldingerstr, D-35033 Marburg, Germany. Konrad@post.med.uni-marburg.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Polyomavirus Transforming* / genetics, metabolism Cell Line, Transformed* Epithelium / physiology* Extracellular Matrix / chemistry Fibronectins / genetics, metabolism Male Membrane Glycoproteins / genetics, metabolism Mesoderm / physiology* Rats Sertoli Cells / cytology*, physiology* Testis / cytology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming; 0/Fibronectins; 0/Membrane Glycoproteins; 0/nidogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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