Document Detail

Rat Sertoli cells express epithelial but also mesenchymal genes after immortalization with SV40.
MedLine Citation:
PMID:  15716129     Owner:  NLM     Status:  MEDLINE    
A new immortal Sertoli cell line from pubertal rat testis was established and characterized. We have generated the clonal line SCIT-C8 expressing established markers for Sertoli cells (SC) like transferrin, clusterin and steel factor/stem cell factor (SCF). Additionally, the immortalized cells express afadin, a protein which is a member of tight and adherens junctions, therefore the cells may be useful for studies of the blood-testis barrier (BTB) in vitro. In contrast to primary SC, the immortalized cells lost expression of androgen receptor and responsiveness to androgens and follicle-stimulating hormone. Surprisingly, we found mRNA expression and protein secretion of the mesenchymal markers, fibronectin and entactin-1, which we also observed for the immortalized SC lines, ASC-17D and 93RS2. In comparison to primary SC, the immortalized cells demonstrated enhanced adhesion in vitro. This correlated with the expression of entactin-1 because adhesion was strongly reduced by antibody perturbation experiments. Additionally, we found the alternatively spliced and primarily muscle cell-specific long variant of TGF-beta2 not only in peritubular cells (PC), but also in the primary and immortalized SC. Furthermore, all immortalized cell lines secreted higher amounts of TGF-beta2 than primary SC. In conclusion, the immortalized SC lines from different developmental stages showed a similar pattern of epithelial and mesenchymal markers.
Lutz Konrad; Marcel Munir Keilani; Andrea Cordes; Elke V?lck-Badouin; Leslie Laible; Martin Albrecht; Heiner Renneberg; Gerhard Aum?ller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-18
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1722     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-04-12     Completed Date:  2005-04-28     Revised Date:  2010-04-12    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  6-14     Citation Subset:  IM    
Department of Urology, Uniklinikum Lahnberge, Baldingerstr, D-35033 Marburg, Germany.
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MeSH Terms
Antigens, Polyomavirus Transforming* / genetics,  metabolism
Cell Line, Transformed*
Epithelium / physiology*
Extracellular Matrix / chemistry
Fibronectins / genetics,  metabolism
Membrane Glycoproteins / genetics,  metabolism
Mesoderm / physiology*
Sertoli Cells / cytology*,  physiology*
Testis / cytology
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Fibronectins; 0/Membrane Glycoproteins; 0/nidogen

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