| Rat parotid gland cell differentiation in three-dimensional culture. | |
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MedLine Citation:
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PMID: 20121592 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of polarized salivary gland cell monolayers has contributed to our understanding of salivary gland physiology. However, these cell models are not representative of glandular epithelium in vivo, and, therefore, are not ideal for investigating salivary epithelial functions. The current study has developed a three-dimensional (3D) cell culture model for rat Par-C10 parotid gland cells that forms differentiated acinar-like spheres on Matrigel. These 3D Par-C10 acinar-like spheres display characteristics similar to differentiated acini in salivary glands, including cell polarization, tight junction (TJ) formation required to maintain transepithelial potential difference, basolateral expression of aquaporin-3 and Na+/K+/2Cl- cotransporter-1, and responsiveness to the muscarinic receptor agonist carbachol that is decreased by the anion channel blocker diphenylamine-2-carboxylic acid or chloride replacement with gluconate. Incubation of the spheres in the hypertonic medium increased the expression level of the water channel aquaporin-5. Further, the proinflammatory cytokines tumor necrosis factor-alpha and interferon-gamma induced alterations in TJ integrity in the acinar-like spheres without affecting individual cell viability, suggesting that cytokines may affect salivary gland function by altering TJ integrity. Thus, 3D Par-C10 acinar-like spheres represent a novel in vitro model to study physiological and pathophysiological functions of differentiated acini. |
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Authors:
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Olga J Baker; David J Schulz; Jean M Camden; Zhongji Liao; Troy S Peterson; Cheikh I Seye; Michael J Petris; Gary A Weisman |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: 16 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-29 Completed Date: 2011-02-10 Revised Date: 2012-04-27 |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
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Languages: eng Pagination: 1135-44 Citation Subset: IM |
Affiliation:
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Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, New York 14214-3092, USA. olgabake@buffalo.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anthranilic Acids / pharmacology Blotting, Western Carbachol / pharmacology Cell Differentiation* Cell Polarity Cells, Cultured Interferon-gamma / pharmacology Microscopy, Confocal Parotid Gland / cytology* Rats Tight Junctions / metabolism Tumor Necrosis Factor-alpha / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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K08 DE017633/DE/NIDCR NIH HHS; K08 DE017633-03/DE/NIDCR NIH HHS; R01 DE017591/DE/NIDCR NIH HHS; R01 DE07389/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anthranilic Acids; 0/Tumor Necrosis Factor-alpha; 51-83-2/Carbachol; 82115-62-6/Interferon-gamma; 91-40-7/fenamic acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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