Document Detail


Rat parotid gland cell differentiation in three-dimensional culture.
MedLine Citation:
PMID:  20121592     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of polarized salivary gland cell monolayers has contributed to our understanding of salivary gland physiology. However, these cell models are not representative of glandular epithelium in vivo, and, therefore, are not ideal for investigating salivary epithelial functions. The current study has developed a three-dimensional (3D) cell culture model for rat Par-C10 parotid gland cells that forms differentiated acinar-like spheres on Matrigel. These 3D Par-C10 acinar-like spheres display characteristics similar to differentiated acini in salivary glands, including cell polarization, tight junction (TJ) formation required to maintain transepithelial potential difference, basolateral expression of aquaporin-3 and Na+/K+/2Cl- cotransporter-1, and responsiveness to the muscarinic receptor agonist carbachol that is decreased by the anion channel blocker diphenylamine-2-carboxylic acid or chloride replacement with gluconate. Incubation of the spheres in the hypertonic medium increased the expression level of the water channel aquaporin-5. Further, the proinflammatory cytokines tumor necrosis factor-alpha and interferon-gamma induced alterations in TJ integrity in the acinar-like spheres without affecting individual cell viability, suggesting that cytokines may affect salivary gland function by altering TJ integrity. Thus, 3D Par-C10 acinar-like spheres represent a novel in vitro model to study physiological and pathophysiological functions of differentiated acini.
Authors:
Olga J Baker; David J Schulz; Jean M Camden; Zhongji Liao; Troy S Peterson; Cheikh I Seye; Michael J Petris; Gary A Weisman
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  16     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2011-02-10     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1135-44     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Carbachol / pharmacology
Cell Differentiation*
Cell Polarity
Cells, Cultured
Interferon-gamma / pharmacology
Microscopy, Confocal
Parotid Gland / cytology*
Rats
Tight Junctions / metabolism
Tumor Necrosis Factor-alpha / pharmacology
ortho-Aminobenzoates / pharmacology
Grant Support
ID/Acronym/Agency:
K08 DE017633/DE/NIDCR NIH HHS; K08 DE017633/DE/NIDCR NIH HHS; R01 DE017591/DE/NIDCR NIH HHS; R01 DE07389/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 0/ortho-Aminobenzoates; 51-83-2/Carbachol; 82115-62-6/Interferon-gamma; 91-40-7/fenamic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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