| Rat CCl(4)-induced cirrhosis plus total portal vein ligation: a new model for the study of hyperammonaemia and brain oedema. | |
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MedLine Citation:
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PMID: 20492509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Animal models used to study hyperammonaemic disorders related to chronic liver disease are unsatisfactory. These animals only develop hyperammonaemia and brain oedema when fed with diets supplemented with amonium acetate. AIM: To develop a novel experimental model of hyperammonaemia and brain oedema in CCl(4)-induced cirrhosis in rats. METHODS: Four groups were studied: rats with sham intervention (S), rats with total portal vein ligation (TPVL), cirrhotic rats (LC), and cirrhotic rats with TPVL (LC+TPVL). When ascites was diagnosed, oral glutamine challenge (OGC) test was performed. Blood, liver, lungs and brain samples were collected to quantify liver function parameters, plasmatic and cerebral ammonia, endotoxaemia, liver and brain histology, brain oedema and portosystemic shunting degree. RESULTS: LC+TPVL rats showed a significant increase in portosystemic shunting when compared with LC group and a significant derangement in liver function when compared with TPVL group. These alterations resulted in a significant increase in plasmatic and brain ammonia concentrations and a higher plasmatic endotoxaemia as compared with others. Similarly, the area under OGC curve was significantly increased in LC+TPVL group as compared with the others, and correlates with portal shunting. Low-grade brain oedema was only observed in LC+TPVL group. All cirrhotic groups showed liver regeneration nodules and type-II Alzheimer astrocytes CONCLUSION: LC+TPVL reproduce the main alterations - portosystemic shunting, plasmatic and cerebral hyperammonaemia and low-grade brain oedema - observed in cirrhotic patients with hepatic encephalopathy. |
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Authors:
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Mireia Miquel; Ramon Bartolí; Gemma Odena; Anna Serafín; Eduard Cabré; Amparo Galan; Ignasi Barba; Joan Córdoba; Ramon Planas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-21 |
Journal Detail:
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Title: Liver international : official journal of the International Association for the Study of the Liver Volume: 30 ISSN: 1478-3231 ISO Abbreviation: Liver Int. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101160857 Medline TA: Liver Int Country: England |
Other Details:
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Languages: eng Pagination: 979-87 Citation Subset: IM |
Affiliation:
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Germans Trias i Pujol Health Science Research Institute, Badalona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascites / etiology Biological Markers / blood Brain / metabolism, pathology, physiopathology Brain Edema / blood, etiology*, pathology, physiopathology Carbon Tetrachloride* Disease Models, Animal Endotoxemia / etiology Hepatic Encephalopathy / blood, etiology*, pathology, physiopathology Hyperammonemia / blood, etiology*, pathology, physiopathology Ligation Liver / blood supply, metabolism, pathology Liver Circulation Liver Cirrhosis / blood, chemically induced, complications*, pathology, physiopathology Male Portal System / physiopathology Portal Vein / surgery* Rats Rats, Sprague-Dawley Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 56-23-5/Carbon Tetrachloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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