| Ras-dependent cell cycle commitment during G2 phase. | |
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MedLine Citation:
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PMID: 11223027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Synchronization used to study cell cycle progression may change the characteristics of rapidly proliferating cells. By combining time-lapse, quantitative fluorescent microscopy and microinjection, we have established a method to analyze the cell cycle progression of individual cells without synchronization. This new approach revealed that rapidly growing NIH3T3 cells make a Ras-dependent commitment for completion of the next cell cycle while they are in G2 phase of the preceding cell cycle. Thus, Ras activity during G2 phase induces cyclin D1 expression. This expression continues through the next G1 phase even in the absence of Ras activity, and drives cells into S phase. |
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Authors:
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M Hitomi; D W Stacey |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
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Title: FEBS letters Volume: 490 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-03-06 Completed Date: 2001-04-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 123-31 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA. hitomi@ccf.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cyclin D1 / antagonists & inhibitors, metabolism G0 Phase G2 Phase* Models, Biological S Phase ras Proteins / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM52271/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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136601-57-5/Cyclin D1; EC 3.6.5.2/ras Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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