Document Detail

Ras-dependent cell cycle commitment during G2 phase.
MedLine Citation:
PMID:  11223027     Owner:  NLM     Status:  MEDLINE    
Synchronization used to study cell cycle progression may change the characteristics of rapidly proliferating cells. By combining time-lapse, quantitative fluorescent microscopy and microinjection, we have established a method to analyze the cell cycle progression of individual cells without synchronization. This new approach revealed that rapidly growing NIH3T3 cells make a Ras-dependent commitment for completion of the next cell cycle while they are in G2 phase of the preceding cell cycle. Thus, Ras activity during G2 phase induces cyclin D1 expression. This expression continues through the next G1 phase even in the absence of Ras activity, and drives cells into S phase.
M Hitomi; D W Stacey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  FEBS letters     Volume:  490     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-06     Completed Date:  2001-04-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  123-31     Citation Subset:  IM    
Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
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MeSH Terms
Cell Cycle*
Cyclin D1 / antagonists & inhibitors,  metabolism
G0 Phase
G2 Phase*
Models, Biological
S Phase
ras Proteins / genetics,  metabolism*
Grant Support
Reg. No./Substance:
136601-57-5/Cyclin D1; EC Proteins

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