Document Detail

Rare Cell Capture in Microfluidic Devices.
MedLine Citation:
PMID:  21532971     Owner:  NLM     Status:  Publisher    
This article reviews existing methods for the isolation, fractionation, or capture of rare cells in microfluidic devices. Rare cell capture devices face the challenge of maintaining the efficiency standard of traditional bulk separation methods such as flow cytometers and immunomagnetic separators while requiring very high purity of the target cell population, which is typically already at very low starting concentrations. Two major classifications of rare cell capture approaches are covered: (1) non-electrokinetic methods (e.g., immobilization via antibody or aptamer chemistry, size-based sorting, and sheath flow and streamline sorting) are discussed for applications using blood cells, cancer cells, and other mammalian cells, and (2) electrokinetic (primarily dielectrophoretic) methods using both electrode-based and insulative geometries are presented with a view towards pathogen detection, blood fractionation, and cancer cell isolation. The included methods were evaluated based on performance criteria including cell type modeled and used, number of steps/stages, cell viability, and enrichment, efficiency, and/or purity. Major areas for improvement are increasing viability and capture efficiency/purity of directly processed biological samples, as a majority of current studies only process spiked cell lines or pre-diluted/lysed samples. Despite these current challenges, multiple advances have been made in the development of devices for rare cell capture and the subsequent elucidation of new biological phenomena; this article serves to highlight this progress as well as the electrokinetic and non-electrokinetic methods that can potentially be combined to improve performance in future studies.
Erica D Pratt; Chao Huang; Benjamin G Hawkins; Jason P Gleghorn; Brian J Kirby
Publication Detail:
Journal Detail:
Title:  Chemical engineering science     Volume:  66     ISSN:  0009-2509     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-5-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9883937     Medline TA:  Chem Eng Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1508-1522     Citation Subset:  -    
Department of Biomedical Engineering, Cornell University, Ithaca NY 14853, United States.
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Grant Support
U54 CA143876-01//NCI NIH HHS; U54 CA143876-02//NCI NIH HHS; U54 CA143876-03//NCI NIH HHS

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