Document Detail


Rapidly alternating transmission mode electron-transfer dissociation and collisional activation for the characterization of polypeptide ions.
MedLine Citation:
PMID:  18396915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cation transmission/electron-transfer reagent anion storage mode electron-transfer ion/ion reactions and beam-type collisional activation of the polypeptide ions are performed in rapid succession in the high-pressure collision cell (Q2) of a quadrupole/time-of-flight tandem mass spectrometer (QqTOF), where the electron-transfer reagent anions are accumulated. Duty cycles for both electron-transfer dissociation (ETD) and collision-induced dissociation (CID) experiments are improved relative to ion trapping approaches since there are no discrete ion storage and reaction steps for ETD experiments and no discrete ion storage step and frequency tuning for CID experiments. For this technique, moderately high resolution and mass accuracy are also obtained due to mass analysis via the TOF analyzer. This relatively simple approach has been demonstrated with a triply charged tryptic peptide, a triply charged tryptic phosphopeptide, and a triply charged tryptic N-linked glycopeptide. For the tryptic peptide, the sequence is identified with more certainty than would be available from a single method alone due to the complementary information provided by these two dissociation methods. Because of the complementary information derived from both ETD and CID dissociation methods, peptide sequence and post-translational modification (PTM) sites for the phosphopeptide are identified. This combined ETD and CID approach is particularly useful for characterizing glycopeptides because ETD generates information about both peptide sequence and locations of the glycosylation sites, whereas CID provides information about the glycan structure.
Authors:
Hongling Han; Yu Xia; Min Yang; Scott A McLuckey
Related Documents :
16108645 - Electron-impact ionization of silicon tetrachloride (sicl4).
21452865 - Fluorescence and spin properties of defects in single digit nanodiamonds.
10407325 - Studies in organic mass spectrometry. part 24dagger electron ionization mass spectra of...
16331895 - Photodissociation study of 1,3-dibromopropane at 234 nm via an ion velocity imaging tec...
20843675 - Facile characterization of polymer fractions from waste electrical and electronic equip...
16553375 - Second hyperpolarizabilities (gamma) of bisimidazole and bistriazole benzenes: diradica...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-04-09
Journal Detail:
Title:  Analytical chemistry     Volume:  80     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-30     Completed Date:  2008-05-20     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3492-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Caseins / chemistry
Cattle
Glycopeptides / chemistry
Horses
Myoglobin / chemistry
Peptides / chemistry*
Plant Lectins / chemistry
Tandem Mass Spectrometry / methods*
Tosylphenylalanyl Chloromethyl Ketone / chemistry
Trypsin / chemistry
Grant Support
ID/Acronym/Agency:
GM 45372/GM/NIGMS NIH HHS; R01 GM045372/GM/NIGMS NIH HHS; R01 GM045372-14/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Caseins; 0/Glycopeptides; 0/Myoglobin; 0/Peptides; 0/Plant Lectins; 0/erythrina lectin; 402-71-1/Tosylphenylalanyl Chloromethyl Ketone; EC 3.4.21.4/Trypsin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Two-dimensional method for time aligning liquid chromatography-mass spectrometry data.
Next Document:  Po(IV) hydration: a quantum chemical study.