Document Detail

Rapid tRNA decay can result from lack of nonessential modifications.
MedLine Citation:
PMID:  16387656     Owner:  NLM     Status:  MEDLINE    
The biological role of many nonessential tRNA modifications outside of the anticodon remains elusive despite their evolutionary conservation. We show here that m7G46 methyltransferase Trm8p/Trm82p acts as a hub of synthetic interactions with several tRNA modification enzymes, resulting in temperature-sensitive growth. Analysis of three double mutants indicates reduced levels of tRNA(Val(AAC)), consistent with a role of the corresponding modifications in maintenance of tRNA levels. Detailed examination of a trm8-delta trm4-delta double mutant demonstrates rapid degradation of preexisting tRNA(Val(AAC)) accompanied by its de-aminoacylation. Multiple copies of tRNA(Val(AAC)) suppress the trm8-delta trm4-delta growth defect, directly implicating this tRNA in the phenotype. These results define a rapid tRNA degradation (RTD) pathway that is independent of the TRF4/RRP6-dependent nuclear surveillance pathway. The degradation of an endogenous tRNA species at a rate typical of mRNA decay demonstrates a critical role of nonessential modifications for tRNA stability and cell survival.
Andrei Alexandrov; Irina Chernyakov; Weifeng Gu; Shawna L Hiley; Timothy R Hughes; Elizabeth J Grayhack; Eric M Phizicky
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular cell     Volume:  21     ISSN:  1097-2765     ISO Abbreviation:  Mol. Cell     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-02     Completed Date:  2006-02-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-96     Citation Subset:  IM    
Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA.
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MeSH Terms
Gene Deletion
Nucleic Acid Conformation
Oligonucleotide Array Sequence Analysis
RNA Processing, Post-Transcriptional*
RNA Stability*
RNA, Transfer, Val / genetics,  metabolism*
Saccharomyces cerevisiae / cytology,  physiology
Saccharomyces cerevisiae Proteins / genetics,  metabolism
Transfer RNA Aminoacylation
tRNA Methyltransferases / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Anticodon; 0/RNA, Transfer, Val; 0/Saccharomyces cerevisiae Proteins; EC 2.1.1.-/tRNA Methyltransferases; EC (guanine-N7-)-methyltransferase
Comment In:
Mol Cell. 2006 Jan 20;21(2):144-5   [PMID:  16427003 ]

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