Document Detail

Rapid spectrophotometric technique for quantifying iron in cells labeled with superparamagnetic iron oxide nanoparticles: potential translation to the clinic.
MedLine Citation:
PMID:  23109392     Owner:  NLM     Status:  MEDLINE    
Labeling cells with superparamagnetic iron oxide (SPIO) nanoparticles provides the ability to track cells by magnetic resonance imaging. Quantifying intracellular iron concentration in SPIO labeled cells would allow for the comparison of agents and techniques used to magnetically label cells. Here we describe a rapid spectrophotometric technique (ST) to quantify iron content of SPIO-labeled cells, circumventing the previous requirement of an overnight acid digestion. Following lysis with 10% sodium dodecyl sulfate (SDS) of magnetically labeled cells, quantification of SPIO doped or labeled cells was performed using commonly available spectrophotometric instrument(s) by comparing absorptions at 370 and 750 nm with correction for turbidity of cellular products to determine the iron content of each sample. Standard curves demonstrated high linear correlation (R(2) = 0.998) between absorbance spectra of iron oxide nanoparticles and concentration in known SPIO-doped cells. Comparisons of the ST with inductively coupled plasma-mass spectroscopy (ICP-MS) or nuclear magnetic resonance relaxometric (R(2)) determinations of intracellular iron contents in SPIO containing samples resulted in significant linear correlation between the techniques (R(2) vs ST, R(2) > 0.992, p < 0.0001; ST vs ICP-MS, R(2) > 0.995, p < 0.0001) with the limit of detection of ST for iron = 0.66 µg ml(-1) for 10(6) cells ml(-1). We have developed a rapid straightforward protocol that does not require overnight acid digestion for quantifying iron oxide content in magnetically labeled cells using readily available analytic instrumentation that should greatly expedite advances in comparing SPIO agents and protocols for labeling cells.
Esmaeel R Dadashzadeh; Matthew Hobson; L Henry Bryant; Dana D Dean; Joseph A Frank
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  Contrast media & molecular imaging     Volume:  8     ISSN:  1555-4317     ISO Abbreviation:  Contrast Media Mol Imaging     Publication Date:    2013 Jan-Feb
Date Detail:
Created Date:  2012-10-30     Completed Date:  2013-04-03     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  101286760     Medline TA:  Contrast Media Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  50-6     Citation Subset:  IM    
Copyright Information:
Published 2012. This article is a U.S. Government work and is in the public domain in the USA.
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MeSH Terms
Ferric Compounds / analysis*,  chemistry,  pharmacology
HeLa Cells
Iron / analysis*,  metabolism
Magnetite Nanoparticles / analysis*,  chemistry
Sensitivity and Specificity
Spectrophotometry, Ultraviolet / methods
Grant Support
Reg. No./Substance:
0/Ferric Compounds; 0/Magnetite Nanoparticles; 1309-37-1/ferric oxide; E1UOL152H7/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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