Document Detail


Rapid, small-scale RNA isolation from tissue culture cells.
MedLine Citation:
PMID:  1718328     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and simple protocol for the isolation of RNA from transfected tissue culture cells is described. The protocol employs a guanidinium thiocyanate/phenol mixture to lyse cells directly from tissue culture plates and extract the total RNA. A total of six simple steps, which can be accomplished within 2.5 hours, are required. The protocol reproducibly yields 20-40 micrograms RNA from 0.5 x 10(6) - 1 x 10(6) cells per sample. The quality of the RNA obtained is sufficient for reverse transcriptase assays such as oligonucleotide-directed primer extension and random-primed cDNA synthesis.
Authors:
W Q Xie; L I Rothblum
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioTechniques     Volume:  11     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-12-06     Completed Date:  1991-12-06     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  324, 326-7     Citation Subset:  IM    
Affiliation:
Weis Center for Research, Geisinger Clinic, Danville, PA 17822-2618.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cricetinae
Genetic Techniques
RNA / isolation & purification*
Rats
Time Factors
Transfection
Chemical
Reg. No./Substance:
63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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