| Rapid, small-scale RNA isolation from tissue culture cells. | |
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MedLine Citation:
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PMID: 1718328 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid and simple protocol for the isolation of RNA from transfected tissue culture cells is described. The protocol employs a guanidinium thiocyanate/phenol mixture to lyse cells directly from tissue culture plates and extract the total RNA. A total of six simple steps, which can be accomplished within 2.5 hours, are required. The protocol reproducibly yields 20-40 micrograms RNA from 0.5 x 10(6) - 1 x 10(6) cells per sample. The quality of the RNA obtained is sufficient for reverse transcriptase assays such as oligonucleotide-directed primer extension and random-primed cDNA synthesis. |
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Authors:
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W Q Xie; L I Rothblum |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: BioTechniques Volume: 11 ISSN: 0736-6205 ISO Abbreviation: BioTechniques Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1991-12-06 Completed Date: 1991-12-06 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8306785 Medline TA: Biotechniques Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 324, 326-7 Citation Subset: IM |
Affiliation:
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Weis Center for Research, Geisinger Clinic, Danville, PA 17822-2618. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cricetinae Genetic Techniques RNA / isolation & purification* Rats Time Factors Transfection |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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