| Rapid, simultaneous quantitation of mono and dioxygenated metabolites of arachidonic acid in human CSF and rat brain. | |
| | |
MedLine Citation:
|
PMID: 19892608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Currently, there are few biomarkers to predict the risk of symptomatic cerebral vasospasm (SV) in subarachnoid hemorrhage (SAH) patients. Mono and dioxygenated arachidonic acid metabolites, involved in the pathogenesis of ischemic injury, may serve as indicators of SV. This study developed a quantitative UPLC-MS/MS method to simultaneously measure hydroxyeicosatetraenoic acid (HETE), dihydroxyeicosatrienoic acid (DiHETrE), and epoxyeicosatrienoic acid (EET) metabolites of arachidonic acid in cerebrospinal fluid (CSF) samples of SAH patients. Additionally, we determined the recovery of these metabolites from polyvinylchloride (PVC) bags used for CSF collection. Linear calibration curves ranging from 0.208 to 33.3 ng/ml were validated. The inter-day and intra-day variance was less than 15% at most concentrations with extraction efficiency greater than 73%. The matrix did not affect the reproducibility and reliability of the assay. In CSF samples, peak concentrations of 8,9-DiHETrE, 20-HETE, 15-HETE, and 12-HETE ranged from 0.293 to 24.9 ng/ml. In rat brain cortical tissue samples, concentrations of 20-, 15-, 12-HETE, 8,9-EET, and 14,15-, 11,12-DiHETrE ranged from 0.57 to 23.99 pmol/g wet tissue. In rat cortical microsomal incubates, all 10 metabolites were measured with formation rates ranging from 0.03 to 7.77 pmol/mg/min. Furthermore, 12-HETE and EET metabolites were significantly altered by contact with PVC bags at all time points evaluated. These data demonstrate that the simultaneous measurement of these compounds in human CSF and rat brain can be achieved with a UPLC-MS/MS system and that this method is necessary for evaluation of these metabolites as potential quantitative biomarkers in future clinical trials. |
| | |
Authors:
|
Tricia M Miller; Mark K Donnelly; Elizabeth A Crago; Dana M Roman; Paula R Sherwood; Michael B Horowitz; Samuel M Poloyac |
Related Documents
:
|
241018 - Cerebrospinal-fluid acid base and electrolyte changes resulting from cerebral anoxia in... 9605468 - Free amino acid distribution inside the first trimester human gestational sac. 3753138 - A sensitive, fast, durable, highly selective method for the determination of amino acid... 120418 - A radiometric microbiologic assay for the biologically active forms at niacin. 2229008 - Purification and characterization of rat pulmonary cytochrome p-450. 3918038 - Cell cycle-dependent changes in arachidonic acid and glycerol metabolism in swiss 3t3 c... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Validation Studies Date: 2009-10-14 |
Journal Detail:
|
Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 877 ISSN: 1873-376X ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-11-16 Completed Date: 2010-03-02 Revised Date: 2011-06-06 |
Medline Journal Info:
|
Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 3991-4000 Citation Subset: IM |
Affiliation:
|
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
8,11,14-Eicosatrienoic Acid
/
analogs & derivatives,
cerebrospinal fluid,
metabolism Animals Arachidonic Acid / cerebrospinal fluid, metabolism* Brain / metabolism* Chromatography, Liquid / methods* Humans Hydroxyeicosatetraenoic Acids / cerebrospinal fluid, metabolism Male Rats Rats, Sprague-Dawley Reference Standards Subarachnoid Hemorrhage / cerebrospinal fluid, metabolism Tandem Mass Spectrometry / methods* Vasospasm, Intracranial / cerebrospinal fluid |
| Grant Support | |
ID/Acronym/Agency:
|
R01 GM073031-04/GM/NIGMS NIH HHS; R01 NR004339-12/NR/NINR NIH HHS; R01 NS052315-05/NS/NINDS NIH HHS; R01NR004339/NR/NINR NIH HHS; R01NS052315/NS/NINDS NIH HHS; S10 RR023461-01/RR/NCRR NIH HHS; S10RR023461/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Hydroxyeicosatetraenoic Acids; 506-32-1/Arachidonic Acid; 7324-41-6/8,11,14-Eicosatrienoic Acid; 79551-86-3/20-hydroxy-5,8,11,14-eicosatetraenoic acid; 81246-85-7/8,9-epoxyeicosatrienoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The rad52-Y66A allele alters the choice of donor template during spontaneous chromosomal recombinati...
Next Document: Narcolepsy with and without cataplexy: An uncommon disabling and unrecognized disease.