| Rapid and simple quantification of bacterial cells by using a microfluidic device. | |
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MedLine Citation:
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PMID: 15691978 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples. |
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Authors:
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Chieko Sakamoto; Nobuyasu Yamaguchi; Masao Nasu |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 71 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-02-04 Completed Date: 2005-04-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 1117-21 Citation Subset: IM |
Affiliation:
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Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques Colony Count, Microbial Culture Media Escherichia coli O157 / isolation & purification* Flow Cytometry / instrumentation, methods* Microfluidics / instrumentation* Microscopy, Fluorescence Organic Chemicals Pseudomonas putida / isolation & purification* Rivers / microbiology Staining and Labeling / methods Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Organic Chemicals; 0/SYBR Green II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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