Document Detail


Rapid and simple detection of the invA gene in Salmonella spp. by isothermal target and probe amplification (iTPA).
MedLine Citation:
PMID:  21291479     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Salmonella spp. are an important cause of food-borne infections throughout world, and the availability of rapid and simple detection techniques is critical for the food industry. Salmonella enterica serovars Enteritidis and Typhimurium cause the majority of human gastroenteritis infections, and there are a reported 40,000 cases of salmonellosis in the USA each year. A novel rapid and simple isothermal target and probe amplification (iTPA) assay that rapidly amplifies target DNA (Salmonella invA gene) using a FRET-based signal probe in an isothermal environment was developed for detection Salmonella spp. in pre-enriched food samples. The assay was able to specifically detect all of 10 Salmonella spp. strains without detecting 40 non-Salmonella strains. The detection limit was 4 x 10(1) CFU per assay. The iTPA assay detected at an initial inoculums level of less than 10 CFU in the pre-enriched food samples (egg yolk, chicken breast, and peanut butter). This detection system requires only a water bath and a fluorometer and has great potential for use as a hand-held device or point-of-care-testing (POCT) diagnostics. The iTPA assay is sensitive and specific and has potential for rapid screening of Salmonella spp. by food industry.
Authors:
Jeong Soon Kim; Moon Sang Jahng; Gang Gweon Lee; Kern Jong Lee; Hong Kwan Chae; Jae Hwa Lee; Joo Hee Lee; Min Hwan Kim
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-3
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Applied Microbiology.
Affiliation:
Food Research & Development Center, Samsung Everland Inc., 553-3 Mabuk-dong, Giheung-gu, Yongin, 446-912, South Korea Food Culture Project Management Office, Samsung Everland Inc., 553-3 Mabuk-dong, Giheung-gu, Yongin, 446-912, South Korea RapleGene Inc., A-207 Kumkang Pentium IT Tower, 333-7 Sangdaewon-dong, Joonwon-gu, Sungnam. 462-901, South Korea.
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