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Rapid and simple DNA extraction method for detection of enterotoxigenic Staphylococcus aureus directly from food samples: Comparison of PCR and LAMP methods.
MedLine Citation:
PMID:  22519925     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  The study describes the development of simple and rapid DNA extraction method in combination with loop-mediated isothermal amplification (LAMP) to detect enterotoxigenic Staphylococcus aureus in food samples. Methods and Results:  In the present study isolation of genomic DNA of enterotoxigenic S. aureus from spiked milk, milk burfi, khoa, sugarcane juice and cooked rice was carried out by boiling the isolated sample pellets for 10 min with 1% Triton X-100. The isolated DNA was evaluated by polymerase chain reaction (PCR) and LAMP method. The LAMP was found to be 100 times more sensitive than PCR. The LAMP assay was very specific for S. aureus and presence of other contaminating bacterial DNAs and food matrix did not interfere or inhibit the LAMP assay. Conclusions:  The template DNA extraction method from food samples, developed in this study is simple, rapid and cost-effective. LAMP was found to be less sensitive to matrix effect of food, compared to PCR. Significance and Impact of the Study:  The method is suitable for direct detection of S. aureus without any enrichment in contaminated food samples and hence finds its application in food safety analysis, in permutation with LAMP. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Sowmya Nagarajappa; Thakur Munna Singh; Manonmani Harave Krishnan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-21
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute (CSIR), Mysore -570 020, Karnataka, India.
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