Document Detail


Rapid separation of gangliosides using strong anion exchanger cartridges.
MedLine Citation:
PMID:  18536509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method utilizing strong anion exchanger cartridges (InertSep SAX) was developed to separate gangliosides. Total lipids extracted from rat brain is able to be rapidly separated into neutral and acidic lipids rapidly. Neutral lipids were passed through the SAX cartridge while acidic lipids adsorbed onto the cartridge and were eluted by chloroform/methanol/4.0 M aqueous ammonium acetate (5:10:1, by volume). Moreover, various kinds of gangliosides (GM1, GD1a, GD1b, GT1b) were separated individually according to their characteristics by elution with increasing concentration of ammnonium acetate (0 - 4.0 M). The gangliosides yield of this procedure was higher than 95%.
Authors:
Tomohisa Kato; Maria Carmelita Z Kasuya; Kenichi Hatanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oleo science     Volume:  57     ISSN:  1347-3352     ISO Abbreviation:  J Oleo Sci     Publication Date:  2008  
Date Detail:
Created Date:  2008-06-09     Completed Date:  2008-09-24     Revised Date:  2009-08-11    
Medline Journal Info:
Nlm Unique ID:  101175339     Medline TA:  J Oleo Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  397-400     Citation Subset:  IM    
Affiliation:
Japan Chemical Innovation Institute, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anion Exchange Resins / chemistry*
Gangliosides / chemistry*,  isolation & purification*
Mass Spectrometry
Rats
Chemical
Reg. No./Substance:
0/Anion Exchange Resins; 0/Gangliosides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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