Document Detail


Rapid, sensitive detection of neurotransmitters at microelectrodes modified with self-assembled SWCNT forests.
MedLine Citation:
PMID:  22823497     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carbon nanotube (CNT) modification of microelectrodes can result in increased sensitivity without compromising time response. However, dip coating CNTs is not very reproducible and the CNTs tend to lay flat on the electrode surface which limits access to the electroactive sites on the ends. In this study, aligned CNT forests were formed using a chemical self-assembly method, which resulted in more exposed CNT ends to the analyte. Shortened, carboxylic acid functionalized single-walled CNTs were assembled from a dimethylformamide (DMF) suspension onto a carbon-fiber disk microelectrode modified with a thin iron hydroxide-decorated Nafion film. The modified electrodes were highly sensitive, with 36-fold higher oxidation currents for dopamine using fast-scan cyclic voltammetry than bare electrodes and 34-fold more current than electrodes dipped in CNTs. The limit of detection (LOD) for dopamine was 17 ± 3 nM at a 10 Hz repetition rate and 65 ± 7 nM at 90 Hz. The LOD at 90 Hz was the same as a bare electrode at 10 Hz, allowing a 9-fold increase in temporal resolution without a decrease in sensitivity. Similar increases were observed for other cationic catecholamine neurotransmitters, and the increases in current were greater than for anionic interferents such as ascorbic acid and 3,4-dihydroxyphenylacetic acid (DOPAC). The CNT forest electrodes had high sensitivity at 90 Hz repetition rate when stimulated dopamine release was measured in Drosophila . The sensitivity, temporal resolution, and spatial resolution of these CNT forest modified disk electrodes facilitate enhanced electrochemical measurements of neurotransmitter release in vivo.
Authors:
Ning Xiao; B Jill Venton
Related Documents :
16820157 - Determination of bromate ion in drinking water by capillary zone electrophoresis with d...
23371487 - Ir-atr chemical sensors based on planar silver halide waveguides coated with an ethylen...
18484187 - Assessment of potential hazards of fluoride contamination in drinking groundwater of an...
22655397 - Concentration and source identification of polycyclic aromatic hydrocarbons and phthali...
19067207 - Comparative impacts of stormwater runoff on water quality of an urban, a suburban, and ...
17723497 - Ph-sensitive diamond field-effect transistors (fets) with directly aminated channel sur...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-08-24
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-18     Completed Date:  2013-03-04     Revised Date:  2013-09-20    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7816-22     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Virginia, Charlottesville, 22904, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3,4-Dihydroxyphenylacetic Acid / chemistry
Animals
Ascorbic Acid / chemistry
Dimethylformamide / chemistry
Dopamine / analysis
Drosophila / metabolism
Electrochemical Techniques*
Ferric Compounds / chemistry
Fluorocarbon Polymers / chemistry
Microelectrodes
Nanotubes, Carbon / chemistry*
Neurotransmitter Agents / analysis*
Oxidation-Reduction
Grant Support
ID/Acronym/Agency:
R01 MH085159/MH/NIMH NIH HHS; R01 MH085159/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Ferric Compounds; 0/Fluorocarbon Polymers; 0/Nanotubes, Carbon; 0/Neurotransmitter Agents; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 2UA751211N/ferric hydroxide; 39464-59-0/perfluorosulfonic acid; 50-81-7/Ascorbic Acid; 68-12-2/Dimethylformamide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Penetrating brain injury caused by nail guns: two case reports and a review of the literature.
Next Document:  On the Determinants of the Conjunction Fallacy: Probability Versus Inductive Confirmation.