| Rapid and sensitive assay of dobutamine in plasma by high-performance liquid chromatography and electrochemical detection. | |
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MedLine Citation:
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PMID: 8106586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive and specific high-performance liquid chromatographic method with electrochemical detection was developed for measuring dobutamine in human plasma samples. Following an external standard method, 0.1 ml of EDTA-glutathione plasma was diluted on ice with 0.2 ml of a 5% trichloracetic acid solution. The mixture was centrifuged, filtered, and 30 microliters were injected. Assessment was done by electrochemical detection. The assay was linear from 1 to 400 ng/ml plasma. For determination of dobutamine we also used a liquid-liquid extraction method routinely applied for plasma catecholamines. Liquid-liquid extraction requires application of 100-1000 microliters of plasma. The standard curve was linear from 0.1 to 600 ng/ml. Absolute recovery of dobutamine was 90 +/- 3% with the liquid-liquid extraction procedure and 91 +/- 3% with the protein precipitation method. For both methods dobutamine was separated on Nova-Pak C18 columns. The mobile phase used was 0.1 molar phosphate buffer-acetonitrile (80:20, v/v) with 1-octanesulfonic acid and triethylamine as ion-pair reagents. The pH was adjusted to 2.7. |
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Authors:
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H Husseini; V Mitrovic; M Schlepper |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 620 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1994-03-23 Completed Date: 1994-03-23 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 164-8 Citation Subset: IM |
Affiliation:
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Max Planck Institute for Physiological and Clinical Research, Kerckhoff-Klinik GmbH, Bad Nauheim, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid
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methods*,
statistics & numerical data Dobutamine / blood* Drug Stability Electrochemistry Humans Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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34368-04-2/Dobutamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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