| Rapid selection of glucose-utilizing variants of the polyhydroxyalkanoate producer Ralstonia eutropha H16 by incubation with high substrate levels. | |
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MedLine Citation:
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PMID: 22044343 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aims: The application of Ralstonia eutropha H16 for producing polyhydroxyalkanoates as bioplastics is limited by the incapability of the bacterium to utilize glucose as a growth substrate. This study aims in characterizing glucose-utilizing strains which arose after incubation with high glucose levels, in comparison to previously published mutants, generated either by mutagenesis or by metabolic engineering. Methods and Results: Cultivations on solid and liquid media showed that application of high substrate concentrations rapidly induced a glucose-positive phenotype. The time span until the onset of growth as well as the frequency of glucose-utilizing colonies were correlated to the initial glucose concentration. All mutants exhibited elevated activities of glucose-6-phosphate dehydrogenase. The glucose-positive phenotype was abolished after deleting genes for the N-acetylglucosamine phosphotransferase system. Conclusions: A procedure is provided for selecting glucose-utilizing R. eutropha H16 in an unprecedented short time period and without any mutagenic treatment. An altered N-acetylglucosamine phosphotransferase system appears to be a common motif in all glucose-utilizing mutants examined so far. Significance and Impact of the Study: The correlation of the applied glucose concentration and the appearance of glucose-utilizing mutants poses questions about the randomness or the specificity of adaptive mutations in general. Furthermore, glucose-adapted strains of R. eutropha H16 could be useful for the production of bioplastics. |
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Authors:
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André Franz; Ruxandra Rehner; Achim Kienle; Hartmut Grammel |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-1 |
Journal Detail:
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Title: Letters in applied microbiology Volume: - ISSN: 1472-765X ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 The Society for Applied Microbiology. |
Affiliation:
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Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106 Magdeburg, Germany Department of Electrical Engineering and Information Technology, Otto von Guericke University Magdeburg, Postfach 4120, 39016 Magdeburg, Germany Magdeburg Centre for Systems Biology (MaCS), Magdeburg, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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