Document Detail


Rapid screening method for quantitation of bacterial cell lipids from whole cells.
MedLine Citation:
PMID:  14529962     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although specific lipids in bacteria can be quantitated, there is still a need to quantitate the total lipid content of a bacterial sample. The sulfo-phospho-vanillin reaction for quantitation of bacterial lipids has significant advantages over traditional methods for screening of engineered mutant strains. In this report we show that this methodology can be used directly on whole cell or homogenized biological material, without any extraction step. The cell components, and most of the reagents used for cell extraction, that were tested did not interfere with the reaction. The screening is based on the observation of physiologic variations using ratios of relative amounts: lipid/DNA and lipid/protein. Our results show that significant differences in those ratios can be detected when there is a modification of the phospholipid content of the cell. The sample manipulation required is minimal and could be automated. Used as a primary screening and/or characterization of engineered mutant strain, the test may lead to further investigation of the nature and distribution of lipids in the cell.
Authors:
Jacques Izard; Ronald J Limberger
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of microbiological methods     Volume:  55     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-07     Completed Date:  2004-02-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  411-8     Citation Subset:  IM    
Affiliation:
New York State Department of Health, Wadsworth Center, David Axelrod Institute for Public Health, P.O. Box 22002, Albany, NY 12201-2002, USA. izard@wadsworth.org
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis,  metabolism
Benzaldehydes / chemistry
DNA, Bacterial / analysis,  metabolism
Escherichia coli / metabolism*
Membrane Lipids / analysis,  metabolism*
Phosphoric Acids / chemistry
Sulfuric Acids / chemistry
Grant Support
ID/Acronym/Agency:
AI34354/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Benzaldehydes; 0/DNA, Bacterial; 0/Membrane Lipids; 0/Phosphoric Acids; 0/Sulfuric Acids; 121-33-5/vanillin; 7664-38-2/phosphoric acid; 7664-93-9/sulfuric acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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