| Rapid-scanning frequency-domain fluorometer with picosecond time resolution. | |
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MedLine Citation:
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PMID: 20490098 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A new frequency-domain fluorometer is described which is capable of operating over a frequency range between 10 MHz and 1 GHz. The instrument employs a mode-locked Ar-ion laser as an 82-MHz repetitionrate excitation source and an inexpensive extracavity acoustooptic light modulator for secondary-frequency generation. The instrument is very rapid and can acquire, in ~10 s, the entire frequency spectrum necessary to determine excited-state lifetimes. Importantly, these frequency spectra are characterized by signal-tonoise ratios of at least 20 for moderate fluorescence signals (10-20 microA detector current). With this new instrument, multiple exponential decays can be easily resolved, and single exponential decays can be determined at a precision (standard deviation) of better than 20 ps. |
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Authors:
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F V Bright; G M Hieftje |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied optics Volume: 26 ISSN: 0003-6935 ISO Abbreviation: Appl Opt Publication Date: 1987 Sep |
Date Detail:
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Created Date: 2010-05-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0247660 Medline TA: Appl Opt Country: United States |
Other Details:
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Languages: eng Pagination: 3526-9 Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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