Document Detail


Rapid and robust detection methods for poison and microbial contamination.
MedLine Citation:
PMID:  22630610     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Real-time on-site monitoring of analytes is currently in high demand for food contamination, water, medicines and ingestible household products that were never tested appropriately. Here we introduce chemical methods for rapid quantification of a wide range of chemical and microbial contaminations using a simple instrument. Within the testing procedure, we used a multi-channel, multisample UV/vis spectrophotometer/fluorometer that employs two frequencies of light simultaneously to interrogate the sample. We present new enzyme- and dye-based methods to detect (di-)ethylene glycol in consumables above 0.1 wt % without interference and alcohols above 1 ppb. Using DNA intercalating dyes we can detect a range of pathogens (Salmonella, E. coli, Cholera and a model for malaria) in water, foods and blood without background signal. We achieved universal scaling independent of pathogen size above 10^4 CFU/ml by taking advantage of the simultaneous measurement at multiple wavelengths. We can detect contaminants directly, without separation, purification, concentration or incubation. Our chemistry is stable to ±1% for more than three weeks without refrigeration, and measurements require less than five minutes.
Authors:
Melanie M Hoehl; Peter Lu; Peter A Sims; Alexander Slocum
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-28
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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