Document Detail

Rapid and reliable high-performance liquid chromatographic method for analysing human plasma serotonin, 5-hydroxyindoleacetic acid, homovanillic acid and 3,4-dihydroxyphenylacetic acid.
MedLine Citation:
PMID:  7691858     Owner:  NLM     Status:  MEDLINE    
The simultaneous measurement of homovanillic acid, 3,4-dihydroxyphenylacetic acid, serotonin and 5-hydroxyindoleacetic acid in human plasma by an ultrafiltration and microbore high-performance liquid chromatography-electrochemical detection technique is established. Conventional preparation of blood is very tedious and time-consuming, but isocratic separation of the analytes in plasma ultrafiltrates using a microbore column could be achieved within 10 min. Hence, theoretically, over 140 analyses can be performed in a working day. The detection limit (signal-to-noise ratio = 3) of this method is about 0.1-0.5 pg per injection for all analytes. The required volume of plasma samples can be less than 100 microliters. Hence, blood loss is minimal, especially in repeated blood sampling. This rapid, simple and sensitive method can, therefore, be used as a routine clinical tool in the simultaneous measurement of plasma homovanillic acid, 3,4-dihydroxyphenylacetic acid, serotonin and 5-hydroxyindoleacetic acid.
F C Cheng; J S Kuo; W H Chang; D J Juang; Y Shih; J S Lai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  617     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-11-15     Completed Date:  1993-11-15     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  227-32     Citation Subset:  IM    
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / blood*
Chromatography, High Pressure Liquid / methods*
Homovanillic Acid / blood*
Hydroxyindoleacetic Acid / blood*
Reproducibility of Results
Serotonin / blood*
Reg. No./Substance:
102-32-9/3,4-Dihydroxyphenylacetic Acid; 333DO1RDJY/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; X77S6GMS36/Homovanillic Acid

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