Document Detail


Rapid and reliable high-performance liquid chromatographic method for analysing human plasma serotonin, 5-hydroxyindoleacetic acid, homovanillic acid and 3,4-dihydroxyphenylacetic acid.
MedLine Citation:
PMID:  7691858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The simultaneous measurement of homovanillic acid, 3,4-dihydroxyphenylacetic acid, serotonin and 5-hydroxyindoleacetic acid in human plasma by an ultrafiltration and microbore high-performance liquid chromatography-electrochemical detection technique is established. Conventional preparation of blood is very tedious and time-consuming, but isocratic separation of the analytes in plasma ultrafiltrates using a microbore column could be achieved within 10 min. Hence, theoretically, over 140 analyses can be performed in a working day. The detection limit (signal-to-noise ratio = 3) of this method is about 0.1-0.5 pg per injection for all analytes. The required volume of plasma samples can be less than 100 microliters. Hence, blood loss is minimal, especially in repeated blood sampling. This rapid, simple and sensitive method can, therefore, be used as a routine clinical tool in the simultaneous measurement of plasma homovanillic acid, 3,4-dihydroxyphenylacetic acid, serotonin and 5-hydroxyindoleacetic acid.
Authors:
F C Cheng; J S Kuo; W H Chang; D J Juang; Y Shih; J S Lai
Related Documents :
15389298 - Amino acid metabolism in the zucker diabetic fatty rat: effects of insulin resistance a...
3042188 - Energy metabolism during chronic endotoxin infusion in the rat, with special reference ...
2753468 - Free amino acid pools in rat tissues throughout the lactational period.
7350838 - Plasma amino acid analysis in the differential diagnosis of jaundice.
5808068 - Fatty acids in the lipids of marine and terrestrial nitrifying bacteria.
12074088 - The synthesis of short- and medium-chain-length poly(hydroxyalkanoate) mixtures from gl...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  617     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-11-15     Completed Date:  1993-11-15     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  227-32     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3,4-Dihydroxyphenylacetic Acid / blood*
Chromatography, High Pressure Liquid / methods*
Electrochemistry
Homovanillic Acid / blood*
Humans
Hydroxyindoleacetic Acid / blood*
Microdialysis
Reproducibility of Results
Serotonin / blood*
Ultrafiltration
Chemical
Reg. No./Substance:
102-32-9/3,4-Dihydroxyphenylacetic Acid; 333DO1RDJY/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; X77S6GMS36/Homovanillic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibition of nitric oxide synthase attenuates the cerebral blood flow response to stimulation of po...
Next Document:  High-performance liquid chromatographic separation of aprotinin-like inhibitors and their determinat...