| Rapid and reliable dideoxy sequencing of double-stranded DNA. | |
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MedLine Citation:
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PMID: 3007296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report a simple and reliable protocol for nucleotide sequencing using the Sanger dideoxy technique on linearized double-stranded DNA molecules with specific oligonucleotide primers. The method is demonstrated for restriction fragments cloned into the plasmid vectors pSP64 and pSP65 using two vector-specific primers, the M13 reverse primer and a new SP6 primer, flanking the multiple cloning site. Template DNA may be prepared by a rapid alkaline lysis procedure. Mild linearization conditions with the appropriate restriction endonuclease avoid the appearance of artifact bands. |
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Authors:
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R G Korneluk; F Quan; R A Gravel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gene Volume: 40 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1985 |
Date Detail:
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Created Date: 1986-04-25 Completed Date: 1986-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 317-23 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant* Genetic Vectors Phosphorus Radioisotopes Plasmids Templates, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/Phosphorus Radioisotopes; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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