Document Detail

Rapid and reliable detection of 11 food-borne pathogens using thin-film biosensor chips.
MedLine Citation:
PMID:  20091028     Owner:  NLM     Status:  MEDLINE    
Traditional methods for identifying food-borne pathogens are time-consuming and laborious, so it is necessary to develop innovative methods for the rapid identification of food-borne pathogens. Here, we report the development of silicon-based optical thin-film biosensor chips for sensitive detection of 11 food-borne pathogens. Briefly, aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip surface, and then, biotinylated polymerase chain reaction (PCR) amplicons were hybridized with the probes. After washing and brief incubation with an antibiotin immunoglobulin G-horseradish peroxidase conjugate and a precipitable horseradish peroxidase substrate, biotinylated chains bound to the probes were visualized as a color change on the chip surface (gold to blue/purple). Highly sensitive and accurate examination of PCR fragment targets can be completed within 30 min. This assay is extremely robust, sensitive, specific, and economical and can be adapted to different throughputs. Thus, a rapid, sensitive, and reliable technique for detecting 11 food-borne pathogens was successfully developed.
Sulan Bai; Jinyi Zhao; Yaochuan Zhang; Wensheng Huang; Shi Xu; Haodong Chen; Liu-Min Fan; Ying Chen; Xing Wang Deng
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-21
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  86     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-06-01     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  983-90     Citation Subset:  IM    
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MeSH Terms
Bacteria / genetics,  isolation & purification*
Biosensing Techniques / methods*
Food Microbiology*
Microarray Analysis / methods*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Grant Support

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