Document Detail


Rapid reduction and removal of HDL- but not LDL-associated cholesteryl ester hydroperoxides by rat liver perfused in situ.
MedLine Citation:
PMID:  8615764     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To test whether high-density lipoproteins (HDL) could aid in the removal in vivo of potentially atherogenic oxidized lipids, we perfused rat liver in situ with buffer supplemented with isolated human HDL containing small amounts of cholesteryl linoleate hydro(pero)xides [CH18:2-O(O)H]. Perfusion resulted in the rapid removal of Ch18:2-O(O)H from HDL with a half-life (t1/2)of 11.4 min., faster than that of unoxidized cholesteryl linoleate, and dependent of the presence of the liver. In addition, the liver enhanced the reduction of Ch18:2-OOH associated with HDL remaining in the perfusate buffer. Perfusion resulted in the release of a hepatic activity that enhanced the reduction of HDL-associated CH18:2-OOH and was resistant to heat treatment. In contrast with the situation with HDL, low-density lipoprotein (LDL)-associated CH18:2-O(O)H were neither removed nor reduced by perfused rat liver within the time course studied, in support of a possible role for HDL in the detoxification of circulating lipid hydroperoxides in vivo.
Authors:
J Christison; A Karjalainen; J Brauman; F Bygrave; R Stocker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  314 ( Pt 3)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-06-03     Completed Date:  1996-06-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  739-42     Citation Subset:  IM    
Affiliation:
Biochemistry Group, Heart Research Institute, Sydney, New South Wales, Australia.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Cholesterol Esters / metabolism*
Cholesterol, HDL / metabolism*
Cholesterol, LDL / metabolism*
Half-Life
Humans
Kinetics
Lipid Peroxides / metabolism*
Liver / metabolism*
Male
Oxidation-Reduction
Perfusion
Rats
Rats, Wistar
Time Factors
Chemical
Reg. No./Substance:
0/Cholesterol Esters; 0/Cholesterol, HDL; 0/Cholesterol, LDL; 0/Lipid Peroxides; 604-33-1/cholesteryl linoleate
Comments/Corrections

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