| Rapid recovery of non-hemolyzed serum and untraumatized cells by using a new method of blood defibrination in vitro. | |
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MedLine Citation:
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PMID: 3760585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Platelet-free cellular elements and non-hemolyzed, chemically unaltered serum are important research components of the cellular immunology laboratory. Both can be recovered from the same peripheral blood sample if it is properly defibrinated. The numbers of cells recovered from heparinized aliquots of blood from healthy donors were not significantly different from the numbers of mononuclear leukocytes, polymorphonuclear leukocytes, and erythrocytes recovered from blood samples which had been mechanically defibrinated in vitro with a stationary, cone-shaped 'TP'-like device which we here describe. Compared with serums obtained from clotted blood, or from blood defibrinated by using glass beads, we found that serums from blood defibrinated with the 'TP'-like device had the lowest detectable levels of hemoglobin, free DNA, or LDH. Serums from TP-defibrinated blood were not different from clotted serum samples with regard to the function of the classical complement pathway, the alternative complement pathway, C4 hemolytic activity, and most serum chemistries. Use of the TP-defibrinator in immunology laboratories is an ideal way to prepare blood for rapid isolation of cellular elements and non-hemolyzed serum from the same sample. |
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Authors:
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H D Kay; H T Petrie; J J Burge; L W Klassen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunological methods Volume: 92 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1986 Sep |
Date Detail:
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Created Date: 1986-11-12 Completed Date: 1986-11-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 251-60 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspartate Aminotransferases
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blood Blood Specimen Collection / instrumentation*, methods Complement System Proteins / physiology DNA / blood Fibrin / deficiency* Hemoglobins / analysis Humans L-Lactate Dehydrogenase / blood |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; 9001-31-4/Fibrin; 9007-36-7/Complement System Proteins; 9007-49-2/DNA; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.6.1.1/Aspartate Aminotransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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