| Rapid quantification of melamine in milk using competitive 1,1'-oxalyldiimidazole chemiluminescent enzyme immunoassay. | |
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MedLine Citation:
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PMID: 20683522 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel competitive 1,1'-oxalyldiimidazole (ODI) chemiluminescent enzyme immunoassay (CLEIA) was developed as a method for rapid and simple screening of melamine in milk. Fat existing in milk acts as an inhibitor in the competitive binding interaction of melamine and anti-melamine in the presence of melamine-conjugated horseradish peroxidase. Thus, the calibration curve and sensitivity of competitive ODI CLEIA for the quantification of melamine in fat free milk were wider and better than those in milk containing fat. However, a centrifuge is not a good method for removing the inhibitor because a portion of the melamine is also removed with the fat. The incubation time (20 min) for the competitive binding interaction of anti-melamine and melamine in 20% milk diluted with PBS buffer of pH 7.4 was longer than that (10 min) in 100% milk even though the sensitivity of the former was better than latter. The limit of detection (1.12 ppb) determined in rapid ODI CLEIA (dynamic range: 3.8-125 ppb) for the quantification of melamine in 20% milk not containing fat was lower than those (6.3 and 9.0 ppb) calculated in relatively time-consuming luminol CLEIA and enzyme-linked immunosorbent assay (ELISA). Also, we expect that ODI-CLEIA (dynamic range: 62.5-2000 ppb) capable of directly quantifying melamine in 100% milk without any pretreatment can be applied as a new and simple method for rapid screening of melamine in milk. |
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Authors:
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JooHee Choi; Young-Teck Kim; Ji Hoon Lee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-04 |
Journal Detail:
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Title: The Analyst Volume: 135 ISSN: 1364-5528 ISO Abbreviation: Analyst Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-17 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: England |
Other Details:
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Languages: eng Pagination: 2445-50 Citation Subset: IM |
Affiliation:
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Langley High School, 6520 Georgetown Pike, McLean, VA 22101, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology Cattle Chemiluminescent Measurements / methods* Horseradish Peroxidase / chemistry, metabolism Imidazoles / chemistry* Immunoenzyme Techniques / methods* Milk / chemistry* Triazines / analysis* |
| Chemical | |
Reg. No./Substance:
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0/1,1'-oxalyldiimidazole; 0/Antibodies; 0/Imidazoles; 0/Triazines; 108-78-1/melamine; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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