Document Detail


Rapid quantifiable assessment of nutritional parameters influencing pediocin production by Pediococcus acidilactici NRRL B5627.
MedLine Citation:
PMID:  18215516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A direct plate bioassay procedure was applied for rapid and quantifiable assessment of the influence of various nutritional parameters on pediocin production by Pediococcus acidilactici NRRL B5627. Solid-state cultivation of the microorganism was done on MRS-based media over 3-and 6-hours incubation periods. Nutritional parameters assessed included the carbon source (glucose, sucrose, fructose, galactose, glycerol), and various salts (NH(4)PO(4), CaCl(2), KH(2)PO(4), MnSO(4).H(2)O). Glucose was found to be the optimal carbon source while glycerol exhibited the most suppressive effect. Using glucose as the carbon source, addition of various salts, in amounts used in liquid media commonly applied in the cultivation of the pediococci, was assessed with respect to bacteriocin production on a per cell basis. Experimental data obtained showed that several nutritional parameters repress pediocin production by P. acidilactici, while the direct plate assay proved to be a good pilot assay prior to conducting more intensive kinetic analysis in liquid cultivation.
Authors:
Sofia Anastasiadou; Maria Papagianni; Ioannis Ambrosiadis; Pavlos Koidis
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Publication Detail:
Type:  Journal Article     Date:  2008-01-22
Journal Detail:
Title:  Bioresource technology     Volume:  99     ISSN:  0960-8524     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-05-26     Completed Date:  2008-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  6646-50     Citation Subset:  IM    
Affiliation:
Department of Hygiene and Technology of Food of Animal Origin, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece.
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MeSH Terms
Descriptor/Qualifier:
Bacteriocins / biosynthesis*
Biological Assay
Culture Media
Kinetics
Pediococcus / metabolism*
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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