Document Detail


Rapid pulsed-field gel electrophoresis protocol for subtyping of Campylobacter jejuni.
MedLine Citation:
PMID:  11326009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial pathogens. Various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, rapid, and robust. PFGE analysis of Campylobacter isolates can be completed in 24 to 30 h using this protocol, whereas the most widely used current protocols require 3 to 4 days to complete. Comparison of PFGE patterns obtained in six laboratories showed that subtyping results obtained using this protocol are highly reproducible.
Authors:
E M Ribot; C Fitzgerald; K Kubota; B Swaminathan; T J Barrett
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  39     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-30     Completed Date:  2001-08-16     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1889-94     Citation Subset:  IM    
Affiliation:
Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA. eyr4@cdc.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Typing Techniques*
Campylobacter Infections / microbiology*
Campylobacter jejuni / classification*,  genetics*
Cattle
Electrophoresis, Gel, Pulsed-Field / methods,  standards
Humans
Time Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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