Document Detail


Rapid protocol for electroporation of Clostridium perfringens.
MedLine Citation:
PMID:  15823401     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.
Authors:
Alena Jirásková; Libor Vítek; Johan Fevery; Tomás Ruml; Pavel Branny
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microbiological methods     Volume:  62     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-04-12     Completed Date:  2005-06-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  125-7     Citation Subset:  IM    
Affiliation:
Institute of Microbiology AS CR, Vídenská 1083, 14220 Prague 4, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Clostridium perfringens / genetics*
Electroporation / methods*
Plasmids / administration & dosage,  genetics
Transformation, Bacterial / genetics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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