Document Detail


Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.
MedLine Citation:
PMID:  8434922     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The polymerase chain reaction was used to selectively amplify sequences within the cholera toxin operon from Vibrio cholerae O1. Oysters, crabmeat, shrimp, and lettuce were seeded with V. cholerae and then homogenized or washed with alkaline peptone water, followed by short-term (6- to 8-h) enrichment. A detection limit of as few as 1 V. cholerae CFU per 10 g of food was obtained with amplification reactions from crude bacterial lysates. The method is extremely rapid and obviates the need for DNA isolation from a variety of complex food matrices.
Authors:
W H Koch; W L Payne; B A Wentz; T A Cebula
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  59     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-17     Completed Date:  1993-03-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  556-60     Citation Subset:  IM    
Affiliation:
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cholera Toxin / analysis*
Food Microbiology*
Fruit
Genes, Bacterial / genetics*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Shellfish
Vegetables
Vibrio cholerae / chemistry,  genetics,  isolation & purification*
Chemical
Reg. No./Substance:
9012-63-9/Cholera Toxin
Comments/Corrections

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