Document Detail


Rapid and orthogonal logic gating with a gibberellin-induced dimerization system.
MedLine Citation:
PMID:  22446836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a newly synthesized gibberellin analog containing an acetoxymethyl group (GA(3)-AM) and its binding proteins, we developed an efficient chemically inducible dimerization (CID) system that is completely orthogonal to existing rapamycin-mediated protein dimerization. Combining the two systems should allow applications that have been difficult or impossible with only one CID system. By using both chemical inputs (rapamycin and GA(3)-AM), we designed and synthesized Boolean logic gates in living mammalian cells. These gates produced output signals such as fluorescence and membrane ruffling on a timescale of seconds, substantially faster than earlier intracellular logic gates. The use of two orthogonal dimerization systems in the same cell also allows for finer modulation of protein perturbations than is possible with a single dimerizer.
Authors:
Takafumi Miyamoto; Robert DeRose; Allison Suarez; Tasuku Ueno; Melinda Chen; Tai-ping Sun; Michael J Wolfgang; Chandrani Mukherjee; David J Meyers; Takanari Inoue
Related Documents :
6198466 - 58,000 dalton intermediate filament proteins of neuronal and nonneuronal origin in the ...
22310046 - Crystal structure of pseudomonas aeruginosa tsi2 reveals a stably folded superhelical a...
22684356 - The role of the ubiquitin-proteasome system in kidney diseases.
10747366 - Regulation of urea transporter proteins in kidney and liver.
17659366 - Xh/pi interactions with the pi system of porphyrin ring in porphyrin-containing proteins.
6198466 - 58,000 dalton intermediate filament proteins of neuronal and nonneuronal origin in the ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-03-25
Journal Detail:
Title:  Nature chemical biology     Volume:  8     ISSN:  1552-4469     ISO Abbreviation:  Nat. Chem. Biol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-18     Completed Date:  2012-06-29     Revised Date:  2014-01-16    
Medline Journal Info:
Nlm Unique ID:  101231976     Medline TA:  Nat Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  465-70     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
PubChem-Substance/134961899;  134961900;  134961901
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Fluorescence
Gibberellins / chemistry*,  pharmacology*
HeLa Cells
Humans
Mice
NIH 3T3 Cells
Protein Multimerization / drug effects*
Sirolimus / chemistry
Grant Support
ID/Acronym/Agency:
DK090868/DK/NIDDK NIH HHS; GM092930/GM/NIGMS NIH HHS; NS072241/NS/NINDS NIH HHS; R01 GM092930/GM/NIGMS NIH HHS; R01 NS072241/NS/NINDS NIH HHS; UL1 RR 025005/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Gibberellins; W36ZG6FT64/Sirolimus
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Rationally designed families of orthogonal RNA regulators of translation.
Next Document:  An engineered eukaryotic protein glycosylation pathway in Escherichia coli.