| Rapid, noninflammatory and PS-dependent phagocytic clearance of necrotic cells. | |
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MedLine Citation:
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PMID: 14502239 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In pathological situations, different modes of cell death are observed, and information on the role and uptake of nonapoptotic corpses is scarce. Here, we modeled two distinct forms of death in human Jurkat T cells treated with staurosporine: classical apoptosis under normal culture conditions and programmed death with necrotic morphology under ATP-depleting conditions (necPCD). When offered to phagocytes, both types of cell corpses (but not heat-killed unscheduled necrotic cells) reduced the release of the proinflammatory cytokine TNF from the macrophages. The necPCD cells were efficiently engulfed by macrophages and microglia, and from mixtures of necPCD and apoptotic cells macrophages preferentially engulfed the necrotic cells. Using a newly developed assay, we demonstrated that phosphatidylserine is translocated to the surface of such necrotic cells. We demonstrate that this can occur independently of calcium signals, and that surface phosphatidylserine is essential for the uptake of necrotic cells by both human macrophages and murine microglia. |
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Authors:
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U A Hirt; M Leist |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell death and differentiation Volume: 10 ISSN: 1350-9047 ISO Abbreviation: Cell Death Differ. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-09-22 Completed Date: 2004-06-07 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9437445 Medline TA: Cell Death Differ Country: England |
Other Details:
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Languages: eng Pagination: 1156-64 Citation Subset: IM |
Affiliation:
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Faculty of Biology, University of Konstanz, X911, D-78457 Konstanz, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Annexin A5 / pharmacology Antibodies, Monoclonal / immunology, pharmacology Antigens, CD14 / immunology Antigens, CD36 / immunology Apoptosis / physiology Calcium / pharmacology, physiology Cell Line Cell Membrane / chemistry Cells, Cultured Escherichia coli / immunology Formaldehyde / pharmacology Humans Inflammation / immunology, metabolism* Ionomycin / pharmacology Jumonji Domain-Containing Histone Demethylases Jurkat Cells / pathology Liposomes / pharmacology Macrophages / cytology, drug effects, metabolism Membrane Lipids / analysis, physiology Mice Microglia / cytology, drug effects, metabolism Microscopy, Confocal Microscopy, Fluorescence Necrosis Oligomycins / pharmacology Oligopeptides / pharmacology Phagocytosis / immunology, physiology* Phosphatidylserines / analysis, physiology* Polymers / pharmacology Receptors, Cell Surface / antagonists & inhibitors Staurosporine / pharmacology Tumor Necrosis Factor-alpha / metabolism, secretion |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Antibodies, Monoclonal; 0/Antigens, CD14; 0/Antigens, CD36; 0/Liposomes; 0/Membrane Lipids; 0/Oligomycins; 0/Oligopeptides; 0/Phosphatidylserines; 0/Polymers; 0/Ptdsr protein, mouse; 0/Receptors, Cell Surface; 0/Tumor Necrosis Factor-alpha; 0/phosphatidylserine receptor; 30525-89-4/paraform; 50-00-0/Formaldehyde; 56092-81-0/Ionomycin; 62996-74-1/Staurosporine; 7440-70-2/Calcium; 91037-65-9/arginyl-glycyl-aspartyl-serine; 93674-97-6/RGES peptide; EC 1.14.11.-/JMJD6 protein, human; EC 1.14.11.-/Jumonji Domain-Containing Histone Demethylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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