Document Detail

Rapid monodisperse microencapsulation of single cells.
MedLine Citation:
PMID:  21096496     Owner:  NLM     Status:  MEDLINE    
A microfluidic device was designed having the ability to continuously produce monodisperse microcapsules with controlled cell loading. The design included stages of inertial focusing, droplet generation, and photopolymerization. Prototype microfluidic devices were fabricated in polydimethylsiloxane (PDMS) to demonstrate each stage using poly(ethylene-glycol)-diacrylate (PEGDA) as the encapsulating material and oil as the droplet-containing medium, creating a water-in-oil emulsion. 10.3-µm-diameter fluorescent polystyrene beads were used as cell simulants. In the first stage, inertial focusing was demonstrated using a straight-channel configuration. In the second stage, droplets with a 60±5µm diameter were generated. In the third stage, successful encapsulation of the beads in hydrogel droplets was verified. This technology can significantly impact a wide research area ranging from cellular therapeutics to single-cell manipulation.
Xiaoxiao Zhang; Aaron T Ohta; David Garmire
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference     Volume:  2010     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2010  
Date Detail:
Created Date:  2010-11-24     Completed Date:  2011-03-17     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6518-21     Citation Subset:  IM    
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MeSH Terms
Capsules / chemistry
Dimethylpolysiloxanes / chemistry
Microfluidic Analytical Techniques / methods*
Polyethylene Glycols / chemistry
Polymers / chemistry
Reg. No./Substance:
0/Capsules; 0/Dimethylpolysiloxanes; 0/Emulsions; 0/Polyethylene Glycols; 0/Polymers; 0/poly(ethylene glycol)diacrylate; 63148-62-9/baysilon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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