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Rapid molecular identification of Listeria species by use of real-time PCR and high-resolution melting analysis.
MedLine Citation:
PMID:  22372942     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Identification of Listeria species via a molecular method is critical for food safety and clinical diagnosis. In this study an assay integrating real-time quantitative PCR (Q-PCR) with high-resolution melting curve analysis was developed and assessed for rapid identification of six Listeria species. The ssrA gene, which encodes a transfer-messenger RNA (tmRNA) and is conserved and common to all bacterial phyla, contains a variable domain in Listeria spp. Therefore, Q-PCR and a high resolution melting profile were applied to characterize this gene. Fifty-three Listeria species and forty-five non-Listeria species were detected using one primer set, with an accuracy of 100% in reference to conventional methods. There was a 93.3% correction rate to thirty artificially contaminated samples. Thus Q-PCR with melting profiling analysis proved able to identify Listeria species accurately. Consequently, this study demonstrates that the assay we developed is a functional tool for rapidly identifying six Listeria species, and has the potential for discriminating novel species food safety and epidemiological research. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Dazhi Jin; Yun Luo; Zheng Zhang; Weijia Fang; Julian Ye; Fang Wu; Gangqiang Ding
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-28
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China; Department of Pathology, Microbiology and Immunology, Vanderbilt University, Tennessee, USA.
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