Document Detail


Rapid, microtubule-dependent fluctuations of the cell margin.
MedLine Citation:
PMID:  9365279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using data automatically acquired by microinterferometry from large numbers of chick fibroblasts, we have detected rapid microfluctuations in the rates of protrusion and retraction of the cell margin which were strongly suppressed by colcemid, nocodazole and taxol. Fluctuations in the rate of retraction of the margin were about twice as powerful as fluctuations in the rate of protrusion. High-frequency fluctuations were also apparent in the cell track and in measures of cell spreading, shape and speed. These rapid fluctuations were also all suppressed by colcemid and nocodazole, sometimes by doses insufficient to disrupt the majority of microtubules. Taxol on the other hand did not suppress fluctuations in direction of the cell track nor fluctuations in the spreading of the cells but it was very effective at suppressing variations in protrusion and retraction and in cell speed and shape. We discovered that much slower, larger-scale variations in protrusion, retraction, spreading, shape and speed resulted from the accumulation of these rapid, microtubule-dependent fluctuations of the cell margin. These large-scale variations in cell behaviour were also suppressed by the same drug treatments that were effective in suppressing the corresponding high-frequency fluctuations. We speculate that a function of microtubules is to enhance the fibroblast's responses to its environment by causing microfluctuations of the cell's margin which give rise to large-scale variations in cell behaviour.
Authors:
G A Dunn; D Zicha; P E Fraylich
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cell science     Volume:  110 ( Pt 24)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-17     Completed Date:  1998-02-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3091-8     Citation Subset:  IM    
Affiliation:
MRC Muscle and Cell Motility Unit, The Randall Institute, King's College London, London WC2B 5RL, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement*
Chick Embryo
Demecolcine / pharmacology
Microtubules / drug effects,  physiology*
Nocodazole / pharmacology
Paclitaxel / pharmacology
Chemical
Reg. No./Substance:
31430-18-9/Nocodazole; 33069-62-4/Paclitaxel; 477-30-5/Demecolcine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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