| Rapid methods for detection and enumeration of Campylobacter spp. in foods. | |
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MedLine Citation:
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PMID: 12180699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Campylobacter spp. are the most commonly reported bacterial cause of acute diarrheal disease in humans throughout the world. Traditional cultural methods for the detection and quantitation of Campylobacterspp. are slow and tedious; therefore, specific, sensitive, and rapid methods for campylobacters are needed to collect sufficient data for risk assessment and food safety policy development. We developed several rapid methods based on polymerase chain reaction (PCR), DNA hybridization, hydrophobic grid membrane filters (HGMFs), and enzyme immunoassays (EIAs). A PCR assay targeting C. jejuni, combined with a simple sample preparation procedure, detects as few as 0.3 most probable number (MPN)/mL C. jejuni in naturally contaminated chicken rinses after 20-24 h enrichment. An HGMF-EIA method using a commercial polyclonal antibody for Campylobacter detects and enumerates thermophilic Campylobacter spp. from spiked chicken rinse and milk, and naturally contaminated chicken rinses. A C. jejuni-specific probe in an HGMF-DNA hybridization protocol specifically detects and quantitates C. jejuni in food samples. A dot-blot EIA combined with an MPN procedure quantitates thermophilic campylobacters from samples that might be difficult to filter through HGMFs. |
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Authors:
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Haiyan Wang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of AOAC International Volume: 85 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2002 Jul-Aug |
Date Detail:
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Created Date: 2002-08-15 Completed Date: 2003-04-07 Revised Date: 2008-03-17 |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 996-9 Citation Subset: IM |
Affiliation:
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Health Canada, Bureau of Microbial Hazards, Health Products and Food Branch, Food Directorate, Ottawa, Ontario. wangha@inspection.gc.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteriological Techniques* Campylobacter / genetics, isolation & purification*, pathogenicity Campylobacter jejuni / genetics, isolation & purification, pathogenicity Chickens / microbiology Colony Count, Microbial DNA, Bacterial / genetics, isolation & purification Food Microbiology* Immunoenzyme Techniques / methods Nucleic Acid Hybridization Polymerase Chain Reaction / methods Risk Assessment |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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