Document Detail


Rapid methods for detection and enumeration of Campylobacter spp. in foods.
MedLine Citation:
PMID:  12180699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Campylobacter spp. are the most commonly reported bacterial cause of acute diarrheal disease in humans throughout the world. Traditional cultural methods for the detection and quantitation of Campylobacterspp. are slow and tedious; therefore, specific, sensitive, and rapid methods for campylobacters are needed to collect sufficient data for risk assessment and food safety policy development. We developed several rapid methods based on polymerase chain reaction (PCR), DNA hybridization, hydrophobic grid membrane filters (HGMFs), and enzyme immunoassays (EIAs). A PCR assay targeting C. jejuni, combined with a simple sample preparation procedure, detects as few as 0.3 most probable number (MPN)/mL C. jejuni in naturally contaminated chicken rinses after 20-24 h enrichment. An HGMF-EIA method using a commercial polyclonal antibody for Campylobacter detects and enumerates thermophilic Campylobacter spp. from spiked chicken rinse and milk, and naturally contaminated chicken rinses. A C. jejuni-specific probe in an HGMF-DNA hybridization protocol specifically detects and quantitates C. jejuni in food samples. A dot-blot EIA combined with an MPN procedure quantitates thermophilic campylobacters from samples that might be difficult to filter through HGMFs.
Authors:
Haiyan Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  85     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2002 Jul-Aug
Date Detail:
Created Date:  2002-08-15     Completed Date:  2003-04-07     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  996-9     Citation Subset:  IM    
Affiliation:
Health Canada, Bureau of Microbial Hazards, Health Products and Food Branch, Food Directorate, Ottawa, Ontario. wangha@inspection.gc.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriological Techniques*
Campylobacter / genetics,  isolation & purification*,  pathogenicity
Campylobacter jejuni / genetics,  isolation & purification,  pathogenicity
Chickens / microbiology
Colony Count, Microbial
DNA, Bacterial / genetics,  isolation & purification
Food Microbiology*
Immunoenzyme Techniques / methods
Nucleic Acid Hybridization
Polymerase Chain Reaction / methods
Risk Assessment
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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