| Rapid measurement of 3J(H N-H alpha) and 3J(N-H beta) coupling constants in polypeptides. | |
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MedLine Citation:
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PMID: 17914658 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present two NMR experiments, (3,2)D HNHA and (3,2)D HNHB, for rapid and accurate measurement of 3J(H N-H alpha) and 3J(N-H beta) coupling constants in polypeptides based on the principle of G-matrix Fourier transform NMR spectroscopy and quantitative J-correlation. These experiments, which facilitate fast acquisition of three-dimensional data with high spectral/digital resolution and chemical shift dispersion, will provide renewed opportunities to utilize them for sequence specific resonance assignments, estimation/characterization of secondary structure with/without prior knowledge of resonance assignments, stereospecific assignment of prochiral groups and 3D structure determination, refinement and validation. Taken together, these experiments have a wide range of applications from structural genomics projects to studying structure and folding in polypeptides. |
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Authors:
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Ravi Pratap Barnwal; Ashok K Rout; Kandala V R Chary; Hanudatta S Atreya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-04 |
Journal Detail:
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Title: Journal of biomolecular NMR Volume: 39 ISSN: 0925-2738 ISO Abbreviation: J. Biomol. NMR Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-02 Completed Date: 2008-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9110829 Medline TA: J Biomol NMR Country: Netherlands |
Other Details:
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Languages: eng Pagination: 259-63 Citation Subset: IM |
Affiliation:
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Department of Chemical Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Hydrogen
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chemistry Hydrogen Bonding Nitrogen / chemistry Nuclear Magnetic Resonance, Biomolecular* Peptides / chemistry* Protein Folding |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 1333-74-0/Hydrogen; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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