| Rapid loss of stress fibers in Chinese hamster ovary cells after hyperthermia. | |
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MedLine Citation:
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PMID: 4038379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was initiated to characterize the effect of hyperthermia (45 degrees) on the distribution of actin stress fibers in Chinese hamster ovary cells using rhodamine-conjugated phalloidin, a probe specific for F-actin. Fluorescent microscopy revealed a rapid loss of stress fibers after immersion in a 45 degrees water bath. After 5-min immersion at 45 degrees, approximately 90% of the cells analyzed did not contain observable stress fibers. Stress fibers were visible after incubation of cells at 37 degrees after heating. The recovery of the appearance of the stress fibers occurred as protein synthesis resumed, and addition of protein synthesis inhibitors following heat treatment blocked the reappearance of these structures. These results support the hypothesis that cytoskeletal components may be a target of hyperthermia, explaining the pleotropic biological effects of heat and, in particular, heat radiosensitization. |
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Authors:
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J R Glass; R G DeWitt; A E Cress |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 45 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1985 Jan |
Date Detail:
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Created Date: 1985-01-25 Completed Date: 1985-01-25 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 258-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Cell Line Cricetinae Cricetulus Cytoskeleton / physiology*, ultrastructure Female Hot Temperature* Kinetics Microscopy, Fluorescence Ovary Protein Biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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CA-30052/CA/NCI NIH HHS; CA-31010/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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