Document Detail

Rapid loss of stress fibers in Chinese hamster ovary cells after hyperthermia.
MedLine Citation:
PMID:  4038379     Owner:  NLM     Status:  MEDLINE    
This study was initiated to characterize the effect of hyperthermia (45 degrees) on the distribution of actin stress fibers in Chinese hamster ovary cells using rhodamine-conjugated phalloidin, a probe specific for F-actin. Fluorescent microscopy revealed a rapid loss of stress fibers after immersion in a 45 degrees water bath. After 5-min immersion at 45 degrees, approximately 90% of the cells analyzed did not contain observable stress fibers. Stress fibers were visible after incubation of cells at 37 degrees after heating. The recovery of the appearance of the stress fibers occurred as protein synthesis resumed, and addition of protein synthesis inhibitors following heat treatment blocked the reappearance of these structures. These results support the hypothesis that cytoskeletal components may be a target of hyperthermia, explaining the pleotropic biological effects of heat and, in particular, heat radiosensitization.
J R Glass; R G DeWitt; A E Cress
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  45     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-01-25     Completed Date:  1985-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  258-62     Citation Subset:  IM    
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MeSH Terms
Actins / metabolism
Cell Line
Cytoskeleton / physiology*,  ultrastructure
Hot Temperature*
Microscopy, Fluorescence
Protein Biosynthesis
Grant Support
Reg. No./Substance:

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