Document Detail


Rapid loss of stress fibers in Chinese hamster ovary cells after hyperthermia.
MedLine Citation:
PMID:  4038379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was initiated to characterize the effect of hyperthermia (45 degrees) on the distribution of actin stress fibers in Chinese hamster ovary cells using rhodamine-conjugated phalloidin, a probe specific for F-actin. Fluorescent microscopy revealed a rapid loss of stress fibers after immersion in a 45 degrees water bath. After 5-min immersion at 45 degrees, approximately 90% of the cells analyzed did not contain observable stress fibers. Stress fibers were visible after incubation of cells at 37 degrees after heating. The recovery of the appearance of the stress fibers occurred as protein synthesis resumed, and addition of protein synthesis inhibitors following heat treatment blocked the reappearance of these structures. These results support the hypothesis that cytoskeletal components may be a target of hyperthermia, explaining the pleotropic biological effects of heat and, in particular, heat radiosensitization.
Authors:
J R Glass; R G DeWitt; A E Cress
Related Documents :
8635489 - Heat shock proteins increase resistance to apoptosis.
18047929 - Heat preconditioning ameliorates hepatocyte viability after cold preservation and rewar...
12902619 - Hsp70 induced by hyperosmotic stress partially protects llc-pk1 cells against nephrotox...
8035809 - Characterization of constitutive hsf2 dna-binding activity in mouse embryonal carcinoma...
15799569 - Heat shock protein 70 delays gentamicin-induced vestibular hair cell death.
20138989 - Heat shock protein 90 interacts with vitamin d receptor in human leukemia cells.
23000969 - Intraepithelial paracrine hedgehog signaling induces the expansion of ciliated cells th...
10730859 - Clonal expansion of epithelial cells from primary human breast carcinoma with 3t3 feede...
16656459 - Reversible swelling and contraction of isolated spinach chloroplasts.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  45     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-01-25     Completed Date:  1985-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  258-62     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cell Line
Cricetinae
Cricetulus
Cytoskeleton / physiology*,  ultrastructure
Female
Hot Temperature*
Kinetics
Microscopy, Fluorescence
Ovary
Protein Biosynthesis
Grant Support
ID/Acronym/Agency:
CA-30052/CA/NCI NIH HHS; CA-31010/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Actins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Erythrocyte mean corpuscular volume during cytotoxic therapy and the risk of secondary leukemia.
Next Document:  Antiinvasive effect of racemic 1-O-octadecyl-2-O-methylglycero-3-phosphocholine on MO4 mouse fibrosa...