Document Detail

Rapid liquid chromatographic determination of 5-hydroxyindoles and dihydroxyphenylacetic acid in cerebrospinal fluid of the rat.
MedLine Citation:
PMID:  6180913     Owner:  NLM     Status:  MEDLINE    
A stainless steel guide was implanted in the anterior third ventricle of the anesthetized rat and an internal needle shorter than the guide was used to continuously collect cerebrospinal fluid (CSF) at a constant outflow of 1 microliter/min. Five microliter samples were injected directly into a liquid chromatographic column. The mobile phase was adjusted for selective separation of 5-hydroxytryptophan (5-HTP), serotonin (5-HT), dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindolacetic acid (5-HIAA). Electrochemical detection with a limit of 0.05 pmol was used, 5-HTP and 5-HT concentrations were in the 10(-8) M range in controls while DOPAC and 5-HIAA were in the 10(-7) and 10(-6) M range. Brain aromatic amino acid decarboxylase inhibition with high doses of benserazide corresponded to an increased CSF level of 5-HTP. Monoamine oxidase inhibition with tranylcypromine resulted in a diminution of DOPAC and 5-HIAA. L-Tryptophan loading associated with monoamine oxidase inhibition induced an increase in CSF level of serotonin. These pharmacologically induced changes in serotonin and dopamine metabolite levels exemplify the usefulness of these CSF determinations as indices of brain function.
K H Le Quan-Bui; J L Elghozi; M A Devynck; P Meyer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of pharmacology     Volume:  81     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  315-20     Citation Subset:  IM    
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / cerebrospinal fluid*
5-Hydroxytryptophan / cerebrospinal fluid
Chromatography, Liquid
Hydroxyindoleacetic Acid / cerebrospinal fluid
Indoles / cerebrospinal fluid*
Phenylacetates / cerebrospinal fluid*
Rats, Inbred Strains
Serotonin / cerebrospinal fluid,  metabolism
Reg. No./Substance:
0/Indoles; 0/Phenylacetates; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 1953-54-4/5-hydroxyindole; 50-67-9/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; 56-69-9/5-Hydroxytryptophan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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