Document Detail


Rapid and label-free cell detection by metal-cluster-decorated carbon nanotube biosensors.
MedLine Citation:
PMID:  19342216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, the use of carbon nanotube biosensors toward alga cell detection was examined. The biosensor devices were fabricated on complete 4 in. wafers by first growing carbon nanotubes (CNTs) and then depositing metal electrodes using a shadow mask. In addition, we decorated the biosensors with metal-clusters resulted in enhancing the sensitivity by 2000-folds and has enabled the detection of streptavidin down to 10 pM concentration. This sensitivity enhancement was attributed to activation of CNT channels due to formation of Schottky junctions between CNTs and metal-clusters. Real-time cell detection has been successfully carried out using the CNT biosensors for two kinds of alga related to brown tides: Aureococcus anophagefferens and BT3. Functionalization of the CNT biosensors with the monoclonal antibody for A. anophagefferens has led to detection at a concentration of 10(4) cells/ml, with sensitivity lower than 10(4) cells/ml projected based on the signal-to-noise ratio of the sensors. Further functionalization with tween 20 led to suppression of non-specific binding of BT3 and enabled label-free and selective detection of A. anophagefferens. These nanobiosensors may find potential applications for environmental monitoring and disease diagnosis.
Authors:
Fumiaki N Ishikawa; Beth Stauffer; David A Caron; Chongwu Zhou
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-03-14
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  24     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-19     Completed Date:  2009-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2967-72     Citation Subset:  IM    
Affiliation:
Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
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MeSH Terms
Descriptor/Qualifier:
Algae, Brown / immunology,  isolation & purification,  pathogenicity
Animals
Antibodies, Monoclonal
Biosensing Techniques / instrumentation*,  methods,  statistics & numerical data
Electrodes
Metals
Microscopy, Electron, Scanning
Nanotubes, Carbon* / ultrastructure
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Metals; 0/Nanotubes, Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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