Document Detail


Rapid isolation in large numbers of intact, viable, individual hair follicles from skin: biochemical and ultrastructural characterization.
MedLine Citation:
PMID:  3782859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid, novel method is described by which large numbers of intact, viable, individual hair follicles may be isolated from rat skin. Follicles are freed from the surrounding connective tissue by shearing, which is effected by repeated cutting with a loosely fitting pair of scissors, and collected individually under liquid using gentle aspiration. Ultrastructural analysis indicates that the follicles are sheared away from the surrounding dermis in the region of the connective tissue capsule which encircles the hair. The follicles appear viable by light and electron microscopy and, within 2 h of isolation, retain the capacity to incorporate [3H]thymidine into DNA and [35S]methionine into proteins as judged by autoradiography. A histologic comparison indicates that the structural integrity of follicles isolated by this new method is significantly superior to those plucked from the animal at the same time. The method affords the isolation of large numbers of hair follicles, without resort to enzyme treatments, suitable for biologic studies in the absence of other skin appendages and dermis.
Authors:
M R Green; C S Clay; W T Gibson; T C Hughes; C G Smith; G E Westgate; M White; T Kealey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  87     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-01-06     Completed Date:  1987-01-06     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  768-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Dermatology / methods*
Dissection
Hair* / metabolism,  ultrastructure
Microscopy, Electron
Microscopy, Electron, Scanning
Rats
Skin*
Specimen Handling / methods*
Waxes
Chemical
Reg. No./Substance:
0/Waxes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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