Document Detail

Rapid indication of multidrug-resistant tuberculosis from liquid cultures using FASTPlaqueTB-RIF, a manual phage-based test.
MedLine Citation:
PMID:  12068986     Owner:  NLM     Status:  MEDLINE    
SETTING: A Mycobacteriology Reference Laboratory in Johannesburg, South Africa. OBJECTIVE: To determine the ability of FASTPlaqueTB-RIF, a rapid bacteriophage-based test, to correctly identify rifampicin susceptibility in clinical strains of Mycobacterium tuberculosis after growth in the Bactec 460 semi-automated liquid culture system. DESIGN: A comparative study of FASTPlaqueTB-RIF and conventional drug susceptibility methods, with selection bias to include sufficient rifampicin-resistant strains. RESULTS: Rifampicin susceptibility results were available for 133 strains of M. tuberculosis. Using the Bactec 460 method, 42 of these strains were rifampicin-resistant and 91 strains were rifampicin-susceptible. A further one strain was found to have a mutation in the rpoB gene which was strongly indicative of rifampicin resistance. Sensitivity, specificity and overall accuracy for the FASTPlaqueTB-RIF were respectively 100%, 98.8% and 99.2% for detection of rifampicin resistance; 95.3% (41/43) of the rifampicin-resistant strains were also resistant to isoniazid (multidrug-resistant). CONCLUSION: FASTPlaqueTB-RIF offers performance comparable to the Bactec 460 method, with results available within 2 days and without the need for specialised equipment. This makes FASTPlaqueTB-RIF a rapid test for rifampicin resistance suitable for widespread application. A combination of the FAST-PlaqueTB-RIF test with semi-automated liquid culture reduces the time required to report susceptibility results, enabling rapid and appropriate management of patients with MDR-TB. Rifampicin resistance was a good predictor of multidrug resistance in this population.
H Albert; A P Trollip; R J Mole; S J B Hatch; L Blumberg
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease     Volume:  6     ISSN:  1027-3719     ISO Abbreviation:  Int. J. Tuberc. Lung Dis.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-18     Completed Date:  2002-11-26     Revised Date:  2006-06-12    
Medline Journal Info:
Nlm Unique ID:  9706389     Medline TA:  Int J Tuberc Lung Dis     Country:  France    
Other Details:
Languages:  eng     Pagination:  523-8     Citation Subset:  IM    
Biotec Laboratories Ltd., Johannesburg, South Africa.
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MeSH Terms
Antibiotics, Antitubercular / pharmacology*
Antitubercular Agents / pharmacology
Cell Culture Techniques
Drug Resistance, Multiple*
Isoniazid / pharmacology
Microbial Sensitivity Tests
Mycobacterium tuberculosis / drug effects*,  pathogenicity*
Reference Values
Reproducibility of Results
Rifampin / pharmacology*
Sensitivity and Specificity
Reg. No./Substance:
0/Antibiotics, Antitubercular; 0/Antitubercular Agents; 13292-46-1/Rifampin; 54-85-3/Isoniazid

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