| Rapid in situ harvesting and cytogenetic analysis of perinatal tissue samples. | |
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MedLine Citation:
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PMID: 8843470 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thirty perinatal solid tissue samples were used in a pilot study to test the efficacy of collagenase disaggregation onto coverslips, open system culture under low O2 conditions, pre-harvest incubation in bromodeoxyuridine (BrdU) and colcemid, and in situ automated harvesting. Following the success of the pilot study, the new method was applied to a further 126 consecutive diagnostic samples making a total of 156. The method reduced average tissue culture times from 17 to 8.7 days (range 2-17), improved success rates from 76 to 88 per cent, and simultaneously increased the resolution of cytogenetic analysis. |
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Authors:
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A M Fisher; A E Cockwell; K J Moore; N M Gregson; P L Campbell; C M Campbell; A Herbert; J C Barber; J A Crolla |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Prenatal diagnosis Volume: 16 ISSN: 0197-3851 ISO Abbreviation: Prenat. Diagn. Publication Date: 1996 Jul |
Date Detail:
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Created Date: 1996-12-31 Completed Date: 1996-12-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8106540 Medline TA: Prenat Diagn Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 615-21 Citation Subset: IM |
Affiliation:
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Wessex Regional Genetics Laboratory, Salisbury District Hospital, Wiltshire, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amnion
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pathology Biopsy Chromosome Banding / methods* Collagenases / metabolism* Culture Techniques / methods Embryonic and Fetal Development / physiology Female Histocytological Preparation Techniques* Humans Muscles / embryology, pathology Ovary / embryology, pathology Pilot Projects Placenta / pathology Pregnancy Skin / embryology, pathology Time Factors Umbilical Cord / pathology |
| Chemical | |
Reg. No./Substance:
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EC 3.4.24.-/Collagenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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