Document Detail

Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors.
MedLine Citation:
PMID:  24807101     Owner:  NLM     Status:  Publisher    
Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen-antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
Mishie Tanino; Toshio Sasajima; Hiroshi Nanjo; Shiori Akesaka; Masami Kagaya; Taichi Kimura; Yusuke Ishida; Masaya Oda; Masataka Takahashi; Taku Sugawara; Toshiaki Yoshioka; Hiroshi Nishihara; Yoichi Akagami; Akiteru Goto; Yoshihiro Minamiya; Shinya Tanaka;
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-8
Journal Detail:
Title:  Brain tumor pathology     Volume:  -     ISSN:  1861-387X     ISO Abbreviation:  Brain Tumor Pathol     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9716507     Medline TA:  Brain Tumor Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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