Document Detail


Rapid immunofluorescence microscopy for diagnosis of melioidosis.
MedLine Citation:
PMID:  15817767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.
Authors:
Vanaporn Wuthiekanun; Varunee Desakorn; Gumphol Wongsuvan; Premjit Amornchai; Allen C Cheng; Bina Maharjan; Direk Limmathurotsakul; Wirongrong Chierakul; Nicholas J White; Nicholas P J Day; Sharon J Peacock
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and diagnostic laboratory immunology     Volume:  12     ISSN:  1071-412X     ISO Abbreviation:  Clin. Diagn. Lab. Immunol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-08     Completed Date:  2005-08-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9421292     Medline TA:  Clin Diagn Lab Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  555-6     Citation Subset:  IM    
Affiliation:
Faculty of Tropical Medicine, Wellcome Trust-Oxford University-Mahidol University Tropical Medicine Research Program, Bangkok, Thailand. lek@tropmedres.ac
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / blood*
Bacteriological Techniques
Burkholderia pseudomallei / immunology,  isolation & purification*
Cell Culture Techniques / methods
Humans
Melioidosis / diagnosis*
Microscopy, Fluorescence / methods*
Predictive Value of Tests
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antigens, Bacterial
Comments/Corrections

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