Document Detail


Rapid identification of extensively and extremely drug resistant tuberculosis from multidrug resistant strains; using PCR-RFLP and PCR-SSCP.
MedLine Citation:
PMID:  23205246     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Resistance in Mycobacterium tuberculosis is caused by mutations in genes encoding drug targets. Investigators have already demonstrated the existence of mutations in codons 88 to 94 in the gyrA gene and also in codons 1400, 1401, and 1483 of rrs gene among extensively and extremely drug resistant tuberculosis (XDR & XXDR-TB) strains. The aim of this study was to identify the XDR and XXDR-TB stains based on their mutational analysis.
MATERIALS AND METHODS: Susceptibility testing against first and second-line anti-tuberculosis drugs was performed by the proportional method. Based on susceptibility results, samples were later analyzed, using PCR-SSCP and PCR-RFLP for detection of mutation in gyrA and rrs genes.
RESULTS: Overall, using proportional method, sixty-three strains (64.9%) were identified as MDR, 8(8.2%) as non-MDR and 26 strains (26.8%) were susceptible. Thirty-one cases (31.9%) were amikacin-resistant and 18 (18.5%) samples were ciprofloxacin-resistant. Using PCR-SSCP and PCR-RFLP, we identified 6(6.2%) and 7(7.2%) resistant strains, respectively. Discrepancy in strains was cross-checked by sequencing. The results showed no mutation in 66.6% and 77.4% of CIP and AMK- resistant strains.
CONCLUSION: Rapid detection of drug-resistant Mycobacterium tuberculosis using molecular techniques could be effective in determining therapeutic regimen and preventing the spread of XDR and MDR TB in the community. We should still keep in mind that a high number of resistant strains may have no mutation in proposed candidate genes.
Authors:
P Tahmasebi; P Farnia; Fm Sheikholslami; Aa Velayati
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Iranian journal of microbiology     Volume:  4     ISSN:  2008-4447     ISO Abbreviation:  Iran J Microbiol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-03     Completed Date:  2012-12-12     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  101518404     Medline TA:  Iran J Microbiol     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  165-70     Citation Subset:  -    
Affiliation:
Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), WHO & UNION Collaborating Centre for TB & Lung Diseases, Darabad, Tehran, Iran.
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