Document Detail


Rapid identification of correctly located intracerebroventricular cannula in the rat.
MedLine Citation:
PMID:  6413786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a radiographic technique to identify a correctly located cannula in the ventricular system of the rat brain during life. A suspension of thorium dioxide was infused into the left lateral ventricle through a cannula placed in the brain under stereotaxic guidance. Correctly located injections were identified by the presence of a distinct thorium shadow on lateral X-ray of the in situ brain. When 6-hydroxydopamine was co-injected with thorium the resultant depletion of striatal dopamine and the changes in a conditioned avoidance responses were correlated with the degree of spreading of thorium through the ventricular system.
Authors:
M P Heyes; S Garnett; G Coates
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  8     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  381-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
3,4-Dihydroxyphenylacetic Acid / analysis
Animals
Catheterization
Cerebral Ventriculography / methods
Corpus Striatum / analysis*
Dopamine / analysis
Hydroxydopamines / administration & dosage*
Hypothalamus / analysis*
Injections, Intraventricular / standards*
Male
Norepinephrine / analysis
Oxidopamine
Rats
Rats, Inbred Strains
Thorium Dioxide / administration & dosage*
Chemical
Reg. No./Substance:
0/Hydroxydopamines; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 1199-18-4/Oxidopamine; 1314-20-1/Thorium Dioxide; 51-41-2/Norepinephrine

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