Document Detail


Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions: application to case of 21/21 Robertsonian translocation.
MedLine Citation:
PMID:  153324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of a combination of transmitted light and epiluminescence after silver and fluorescent staining of chromosome preparations makes it possible to achieve simultaneous visualization of silver-stained NORs and flourescent chromosomes. This technique permits exact localization of silver precipitates on normal and BrdU-substituted chromosomes. After previous silver impregnation, fluorescent staining by actinomycin-daunomycin-DAPI was used to induce a banding pattern that enables identification of specific chromosomes while observing silver-stained NORs at the same time. Application of this method to a Down's syndrome patient revealed a 21/21 Robertsonian translocation with NOR'S eliminated.
Authors:
C H Buys; J Osinga; W L Gouw; G J Anders
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  44     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1978 Oct 
Date Detail:
Created Date:  1979-03-24     Completed Date:  1979-03-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  173-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleolus*
Cells, Cultured
Chromosome Banding
Chromosomes, Human / ultrastructure*
Chromosomes, Human, 21-22 and Y
Down Syndrome / genetics*
Humans
Lymphocytes / ultrastructure
Silver
Translocation, Genetic
Chemical
Reg. No./Substance:
7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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