Document Detail

Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions: application to case of 21/21 Robertsonian translocation.
MedLine Citation:
PMID:  153324     Owner:  NLM     Status:  MEDLINE    
The use of a combination of transmitted light and epiluminescence after silver and fluorescent staining of chromosome preparations makes it possible to achieve simultaneous visualization of silver-stained NORs and flourescent chromosomes. This technique permits exact localization of silver precipitates on normal and BrdU-substituted chromosomes. After previous silver impregnation, fluorescent staining by actinomycin-daunomycin-DAPI was used to induce a banding pattern that enables identification of specific chromosomes while observing silver-stained NORs at the same time. Application of this method to a Down's syndrome patient revealed a 21/21 Robertsonian translocation with NOR'S eliminated.
C H Buys; J Osinga; W L Gouw; G J Anders
Related Documents :
1491374 - Tissue expanders in the gut: a histological and angiographic study.
61844 - Detection of nucleolus organizer regions in chromosomes of human, chimpanzee, gorilla, ...
21594604 - Prenatal diagnosis of 45,x/46,xy mosaicism with cleft lip and epispadias.
2427434 - Intensity heteromorphisms of human chromosome 15p by da/dapi technique.
19069914 - Association of h. pylori virulence genes caga, vaca and ureab with ulcer and nonulcer d...
20644634 - Stable morphology, but dynamic internal reorganisation, of interphase human chromosomes...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  44     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1978 Oct 
Date Detail:
Created Date:  1979-03-24     Completed Date:  1979-03-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  173-80     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Nucleolus*
Cells, Cultured
Chromosome Banding
Chromosomes, Human / ultrastructure*
Chromosomes, Human, 21-22 and Y
Down Syndrome / genetics*
Lymphocytes / ultrastructure
Translocation, Genetic
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  On the peripheral location of the Y chromosome.
Next Document:  Inversions and other unusual heteromorphisms detected by C-banding.