Document Detail


Rapid homogeneous detection of biological assays using magnetic modulation biosensing system.
MedLine Citation:
PMID:  20616784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A magnetic modulation biosensing system (MMB) [1,2] rapidly and homogeneously detected biological targets at low concentrations without any washing or separation step. When the IL-8 target was present, a 'sandwich'-based assay attached magnetic beads with IL-8 capture antibody to streptavidin coupled fluorescent protein via the IL-8 target and a biotinylated IL-8 antibody. The magnetic beads are maneuvered into oscillatory motion by applying an alternating magnetic field gradient through two electromagnetic poles. The fluorescent proteins, which are attached to the magnetic beads are condensed into the detection area and their movement in and out of an orthogonal laser beam produces a periodic fluorescent signal that is demodulated using synchronous detection. The magnetic modulation biosensing system was previously used to detect the coding sequences of the non-structural Ibaraki virus protein 3 (NS3) complementary DNA (cDNA) [2]. The techniques that are demonstrated in this work for external manipulation and condensation of particles may be used for other applications, e.g. delivery of magnetically-coupled drugs in-vivo or enhancing the contrast for in-vivo imaging applications.
Authors:
Amos Danielli; Noga Porat; Marcelo Ehrlich; Ady Arie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media     Date:  2010-06-13
Journal Detail:
Title:  Journal of visualized experiments : JoVE     Volume:  -     ISSN:  1940-087X     ISO Abbreviation:  J Vis Exp     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-07-29     Revised Date:  2012-09-24    
Medline Journal Info:
Nlm Unique ID:  101313252     Medline TA:  J Vis Exp     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Washington University in St. Louis, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / chemistry,  immunology
Biosensing Techniques / instrumentation,  methods*
Biotin / chemistry
Fluorescent Dyes / chemistry
Immunoassay / instrumentation,  methods*
Interleukin-8 / analysis*,  chemistry,  immunology
Magnetics / instrumentation,  methods*
Streptavidin / chemistry
Chemical
Reg. No./Substance:
0/Antibodies; 0/Fluorescent Dyes; 0/Interleukin-8; 58-85-5/Biotin; 9013-20-1/Streptavidin
Comments/Corrections

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